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Human P2RX7 / P2X purinoceptor 7 ELISA Kit

SKU:
HUFI01137
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q99572
Sensitivity:
0.188ng/ml
Range:
0.313-20ng/ml
ELISA Type:
Sandwich
Synonyms:
P2RX7, P2X purinoceptor 7, P2X7, Purinergic receptor, ATP receptor, P2Z receptor
Reactivity:
Human
Research Area:
Signal Transduction
$719
Frequently bought together:

Description

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Human P2RX7/P2X purinoceptor 7 ELISA Kit

The Human P2RX7 (P2X purinoceptor 7) ELISA Kit is a powerful tool for the accurate quantification of P2RX7 levels in human samples. With a high level of sensitivity and specificity, this kit delivers reliable and reproducible results, making it perfect for a variety of research applications.P2RX7 is a key protein involved in purinergic signaling, playing a critical role in inflammation, immunity, and cell death. Dysregulation of P2RX7 has been linked to various diseases such as cancer, autoimmune disorders, and neurodegenerative conditions, highlighting its importance as a potential therapeutic target and biomarker.

With the Human P2RX7 ELISA Kit, researchers can study the role of P2RX7 in disease pathogenesis, identify potential drug targets, and monitor treatment efficacy. Trust in this kit for accurate and efficient detection of P2RX7 levels, advancing your research efforts in this important area of study.

Product Name:Human P2RX7 / P2X purinoceptor 7 ELISA Kit
Product Code:HUFI01137
Size:96 Assays
Alias:P2RX7, P2X purinoceptor 7, P2X7, Purinergic receptor, ATP receptor, P2Z receptor
Detection method:Sandwich ELISA, Double Antibody
Application:This immunoassay kit allows for the in vitro quantitative determination of Human P2RX7 concentrations in serum plasma and other biological fluids.
Sensitivity:0.188ng/ml
Range:0.313-20ng/ml
Storage:4°C for 6 months
Note:For Research Use Only
Recovery:Matrices listed below were spiked with certain level of Human P2RX7 and the recovery rates were calculated by comparing the measured value to the expected amount of Human P2RX7 in samples.
MatrixRecovery range(%)Average(%)
serum(n=5)87-10294
EDTA plasma(n=5)92-10599
UFH plasma(n=5)94-10598
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human P2RX7 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample1:21:41:8
serum(n=5)87-104%85-101%87-102%
EDTA plasma(n=5)88-96%88-99%84-99%
UFH plasma(n=5)85-98%84-96%85-98%
CV(%):Intra-Assay: CV<8%
Inter-Assay: CV<10%
ComponentQuantityStorage
ELISA Microplate (Dismountable)8×12 strips4°C for 6 months
Lyophilized Standard24°C/-20°C
Sample/Standard Dilution Buffer20ml4°C
Biotin-labeled Antibody(Concentrated)120ul4°C (Protect from light)
Antibody Dilution Buffer10ml4°C
HRP-Streptavidin Conjugate(SABC)120ul4°C (Protect from light)
SABC Dilution Buffer10ml4°C
TMB Substrate10ml4°C (Protect from light)
Stop Solution10ml4°C
Wash Buffer(25X)30ml4°C
Plate Sealer5-

Other materials and equipment required:

  • Microplate reader with 450 nm wavelength filter
  • Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
  • Incubator
  • Deionized or distilled water
  • Absorbent paper
  • Buffer resevoir
UniprotQ99572
UniProt Protein Function:P2X7: a receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis by macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Alternatively spliced variants encoding different isoforms have been identified. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. Phosphorylation results in its inactivation.
UniProt Protein Details:Protein type:Channel, ligand-gated; Membrane protein, integral; Membrane protein, multi-pass; Receptor, misc.

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: cytoplasm; integral to nuclear inner membrane; integral to plasma membrane; membrane; plasma membrane

Molecular Function:ATP binding; ATP-gated cation channel activity; lipopolysaccharide binding; protein binding; purinergic nucleotide receptor activity; receptor binding

Biological Process: bleb formation; blood coagulation; cell surface receptor linked signal transduction; membrane depolarization; negative regulation of bone resorption; negative regulation of cell volume; negative regulation of MAPKKK cascade; phospholipid scrambling; pore complex biogenesis; positive regulation of bone mineralization; positive regulation of cytolysis; positive regulation of cytoskeleton organization and biogenesis; positive regulation of glycolysis; positive regulation of interleukin-1 beta secretion; regulation of sodium ion transport; response to ATP; sensory perception of pain

Disease: Leukemia, Chronic Lymphocytic

NCBI Summary:The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants have been identified, most of which fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq, Jul 2010]
UniProt Code:Q99572
NCBI GenInfo Identifier:395398617
NCBI Gene ID:5027
NCBI Accession:Q99572.4
UniProt Secondary Accession:Q99572,O14991, Q4VKH8, Q4VKH9, Q4VKI0, Q4VKI1, Q4VKI2 A8K2Z0, E7EMK6, F5H6P2, F5H7E8, F8W951,
UniProt Related Accession:Q99572
Molecular Weight:69kDa
NCBI Full Name:P2X purinoceptor 7
NCBI Synonym Full Names:purinergic receptor P2X 7
NCBI Official Symbol:P2RX7
NCBI Official Synonym Symbols:P2X7
NCBI Protein Information:P2X purinoceptor 7
UniProt Protein Name:P2X purinoceptor 7
UniProt Synonym Protein Names:ATP receptor; P2Z receptor; Purinergic receptor
Protein Family:P2X purinoceptor
UniProt Gene Name:P2RX7

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Before adding to wells, equilibrate the SABC working solution and TMB substrate for at least 30 min at 37°C. When diluting samples and reagents, they must be mixed completely and evenly. It is recommended to plot a standard curve for each test.

StepProtocol
1.Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample in duplicate. Wash plate 2 times before adding standard, sample and control (zero) wells!
2.Aliquot 0.1ml standard solutions into the standard wells.
3.Add 0.1 ml of Sample / Standard dilution buffer into the control (zero) well.
4.Add 0.1 ml of properly diluted sample ( Human serum, plasma, tissue homogenates and other biological fluids.) into test sample wells.
5.Seal the plate with a cover and incubate at 37 °C for 90 min.
6.Remove the cover and discard the plate content, clap the plate on the absorbent filter papers or other absorbent material. Do NOT let the wells completely dry at any time. Wash plate X2.
7.Add 0.1 ml of Biotin- detection antibody working solution into the above wells (standard, test sample & zero wells). Add the solution at the bottom of each well without touching the side wall.
8.Seal the plate with a cover and incubate at 37°C for 60 min.
9.Remove the cover, and wash plate 3 times with Wash buffer. Let wash buffer rest in wells for 1 min between each wash.
10.Add 0.1 ml of SABC working solution into each well, cover the plate and incubate at 37°C for 30 min.
11.Remove the cover and wash plate 5 times with Wash buffer, and each time let the wash buffer stay in the wells for 1-2 min.
12.Add 90 µl of TMB substrate into each well, cover the plate and incubate at 37°C in dark within 10-20 min. (Note: This incubation time is for reference use only, the optimal time should be determined by end user.) And the shades of blue can be seen in the first 3-4 wells (with most concentrated standard solutions), the other wells show no obvious color.
13.Add 50 µl of Stop solution into each well and mix thoroughly. The color changes into yellow immediately.
14. Read the O.D. absorbance at 450 nm in a microplate reader immediately after adding the stop solution.

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample TypeProtocol
SerumIf using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

PlasmaCollect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.
Urine & Cerebrospinal FluidCollect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
Cell culture supernatantCollect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
Cell lysatesSolubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Tissue homogenatesThe preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.
Tissue lysatesRinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Breast MilkCollect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

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