The Human p27 (p27 protein) ELISA Kit is specifically designed for the precise measurement of p27 protein levels in human serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.p27 is a key regulator of cell cycle progression and plays a vital role in controlling cell growth and proliferation. Dysregulation of p27 expression has been linked to various diseases, including cancer, indicating its importance as a biomarker for disease research and therapeutic development.
With its high-performance capabilities, the Human p27 ELISA Kit is an essential tool for researchers seeking to investigate the role of p27 protein in disease mechanisms and potential treatment strategies. Get reliable and valuable insights into cellular processes with this innovative ELISA kit from Assay Genie.
Product Name:
Human P27 (P27 Protein) ELISA Kit
SKU:
HUES02035
Target:
Human P27 (P27 Protein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human P27. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human P27 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human P27, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human P27. You can calculate the concentration of Human P27 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
90-103
92-107
Average (%)
92
95
98
1:4
Range (%)
86-99
80-90
88-101
Average (%)
92
86
93
1:8
Range (%)
92-104
81-96
82-96
Average (%)
97
88
89
1:16
Range (%)
91-107
84-96
86-97
Average (%)
98
91
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
93
EDTA plasma (n=5)
86-101
92
Cell culture media (n=5)
91-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.7
116.19
482.88
49.72
110.38
466.67
Standard deviation
3.49
5.29
18.54
2.64
5.2
24.64
C V (%)
6.88
4.55
3.84
5.31
4.71
5.28
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human P27 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human P27 in samples. No significant cross-reactivity or interference between Human P27 and analogues was observed.
