Human PⅠCP (Procollagen Ⅰ C-Terminal Propeptide) ELISA Kit
The Procollagen Ⅰ C-Terminal Propeptide (PⅠCP) ELISA Kit is meticulously crafted for the precise quantitative measurement of human PⅠCP levels in diverse biological samples.
PⅠCP is a key biomarker that provides valuable insights into collagen synthesis processes and is particularly relevant in the context of bone health assessment, collagen-related disorders, and osteoporosis diagnosis and monitoring. Monitoring PⅠCP levels aids in evaluating bone formation dynamics and predicting bone metabolic activities, making it an essential marker in bone metabolism studies. Our PⅠCP ELISA Kit ensures exceptional sensitivity and specificity, guaranteeing accurate and reproducible results crucial for your scientific investigations. With stringent quality control procedures in place, this kit promises reliable performance and user-friendly protocols, rendering it ideal for both research and clinical applications. Rely on Assay Genie's PⅠCP ELISA Kit for precise and dependable quantification of this pivotal bone health biomarker in your studies.
Product Name:
Human PⅠCP (Procollagen Ⅰ C-Terminal Propeptide) ELISA Kit
SKU:
AEES00066
Target:
Human PⅠCP (Procollagen Ⅰ C-Terminal Propeptide)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PⅠCP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PⅠCP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PⅠCP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PⅠCP. You can calculate the concentration of Human PⅠCP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-99
94-109
91-104
Average (%)
93
102
98
1:4
Range (%)
96-109
86-95
89-99
Average (%)
102
90
92
1:8
Range (%)
95-106
89-100
94-108
Average (%)
101
92
100
1:16
Range (%)
94-105
91-101
89-99
Average (%)
97
98
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-98
91
EDTA plasma (n=5)
93-104
100
Cell culture media (n=5)
96-102
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.34
5.03
22.39
2.15
6.09
21.5
Standard deviation
0.13
0.24
0.92
0.13
0.29
1.12
C V (%)
5.56
4.77
4.11
6.05
4.76
5.21
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PⅠCP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PⅠCP in samples. No significant cross-reactivity or interference between Human PⅠCP and analogues was observed.
