The Human OXB (Orexin B) ELISA Kit is a cutting-edge assay developed for the sensitive and specific measurement of Orexin B levels in human samples. Orexin B, a neuropeptide associated with the regulation of sleep-wake cycles and energy homeostasis, plays a crucial role in modulating various physiological processes, including arousal, appetite, and metabolism.
This ELISA kit provides researchers with a powerful tool to investigate Orexin B levels and its functions in human biological samples. With high sensitivity and specificity, the Human OXB ELISA Kit enables accurate quantification of Orexin B, allowing researchers to explore the role of Orexin B in sleep regulation, energy balance, and other neurophysiological functions. By precisely measuring Orexin B levels, researchers can gain valuable insights into its involvement in sleep disorders, metabolic diseases, and other conditions influenced by neuropeptide signaling pathways. Designed to deliver reliable and reproducible results, the Human OXB ELISA Kit offers robust performance and user-friendly protocols, ensuring exceptional accuracy in Orexin B quantitation. Whether studying sleep biology, metabolic regulation, or neuroendocrine signaling, this ELISA kit provides a valuable resource for researchers investigating Orexin B-related mechanisms and their implications in human health and disease.
Product Name:
Human OXB (Orexin B) ELISA Kit
SKU:
AEES00667
Target:
Human OXB (Orexin B)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.5h
Sensitivity:
0.23 ng/mL
Detection range:
0.39-25 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with OXB. During the reaction, OXB in the sample or standard competes with a fixed amount of OXB on the solid phase supporter for sites on the Biotinylated Detection Ab specific to OXB. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of OXB in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-107
100-112
89-101
Average (%)
102
106
95
1:4
Range (%)
89-100
88-101
99-116
Average (%)
94
95
106
1:8
Range (%)
84-98
87-100
97-111
Average (%)
91
94
104
1:16
Range (%)
91-102
90-103
96-110
Average (%)
96
97
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-104
95
EDTA plasma (n=5)
94-110
101
Cell culture media (n=5)
95-109
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.3
3.1
10.4
1.3
2.9
11.2
Standard deviation
0.1
0.2
0.5
0.1
0.2
0.6
C V (%)
7.69
6.45
4.81
7.69
6.9
5.36
Sample type &Sample volume:
Serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of OXB concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes OXB in samples. No significant cross-reactivity or interference between OXB and analogues was observed.
