The Human OXA (Orexin-A) ELISA Kit is specifically designed for the precise measurement of orexin-A levels in human samples such as serum, plasma, and cell culture supernatants. This kit is characterized by its high sensitivity and specificity, ensuring accurate and consistent results, making it an excellent tool for various research applications.Orexin-A, also known as hypocretin-1, is a neuropeptide that plays a crucial role in regulating wakefulness, appetite, and energy metabolism.
Dysregulation of orexin-A has been implicated in conditions such as narcolepsy, obesity, and sleep disorders, making it a valuable biomarker for studying these disorders and developing potential interventions.By utilizing the Human OXA ELISA Kit, researchers can gain valuable insights into the role of orexin-A in health and disease, facilitating the advancement of scientific knowledge and the development of novel therapeutic strategies.
Product Name:
Human OXA (Orexin A) ELISA Kit
SKU:
HUES02121
Target:
Human OXA (Orexin A)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with OXA. During the reaction, OXA in the sample or standard competes with a fixed amount of OXA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to OXA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of OXA in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-116
91-103
100-111
Average (%)
106
96
106
1:4
Range (%)
90-102
93-106
94-111
Average (%)
96
100
101
1:8
Range (%)
88-101
93-109
91-107
Average (%)
93
99
99
1:16
Range (%)
86-97
94-107
98-113
Average (%)
91
99
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-106
97
EDTA plasma (n=5)
93-111
101
Cell culture media (n=5)
88-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
188.5
568.1
1493.0
170.8
598.4
1389.6
Standard deviation
10.2
25.6
62.7
8.5
31.7
48.6
C V (%)
5.41
4.51
4.2
4.98
5.3
3.5
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of OXA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes OXA in samples. No significant cross-reactivity or interference between OXA and analogues was observed.
