null

Human Ovarian Cancer Immuno Reactive Antigen Domain Containing Protein 2 (OCIAD2) ELISA Kit

SKU:
HUDL02128
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q56VL3
Sensitivity:
0.112ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
OCIA Domain Containing 2,Ovarian carcinoma immunoreactive antigen-like protein
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Human Ovarian Cancer Immuno Reactive Antigen Domain Containing Protein 2 (OCIAD2) ELISA Kit

The Human Ovarian Cancer Immuno-reactive Antigen Domain Containing Protein 2 (OCIAD2) ELISA Kit is a cutting-edge tool for the precise measurement of OCIAD2 levels in human samples. This advanced kit offers unparalleled sensitivity and specificity, ensuring accurate and consistent results for various research purposes.OCIAD2 is a key protein associated with ovarian cancer, playing a vital role in tumor growth and progression. By detecting OCIAD2 levels, researchers can gain valuable insights into the mechanisms underlying ovarian cancer development, leading to the potential identification of novel therapeutic targets and treatment strategies.

With its high-quality components and user-friendly protocols, the OCIAD2 ELISA Kit is a valuable resource for scientists and clinicians alike, seeking to further understand and combat ovarian cancer.Unlock the potential of OCIAD2 research with this innovative ELISA kit, paving the way for advancements in cancer biology and personalized medicine.

Product Name:Human Ovarian Cancer Immuno Reactive Antigen Domain Containing Protein 2 (OCIAD2) ELISA Kit
Product Code:HUDL02128
Size:96 Assays
Target:OCIAD2
Synonyms:OCIA Domain Containing 2, Ovarian carcinoma immunoreactive antigen-like protein
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products