Human OTUB1 Recombinant Protein (RPPB4174)
- SKU:
- RPPB4174
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q96FW1
Description
| Product Name: | Human OTUB1 Recombinant Protein |
| Product Code: | RPPB4174 |
| Size: | 25µg |
| Species: | Human |
| Target: | OTUB1 |
| Synonyms: | Ubiquitin thioesterase OTUB1, Otubain-1, OTU domain-containing ubiquitin aldehyde-binding protein 1, Ubiquitin-specific-processing protease OTUB1, Deubiquitinating enzyme OTUB1, OTUB1, OTB1, OTU1, HSPC263, MGC4584, FLJ20113, FLJ40710, MGC111158. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The OTUB1 solution contains 20mM Tris buffer (pH 8.0) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MAAEEPQQQK QEPLGSDSEG VNCLAYDEAI MAQQDRIQQE IAVQNPLVSE RLELSVLYKE YAEDDNIYQQ KIKDLHKKYS YIRKTRPDGN CFYRAFGFSH LEALLDDSKE LQRFKAVSAK SKEDLVSQGF TEFTIEDFHN TFMDLIEQVE KQTSVADLLA SFNDQSTSDY LVVYLRLLTS GYLQRESKFF EHFIEGGRTV KEFCQQEVEP MCKESDHIHI IALAQALSVS IQVEYMDRGE GGTTNPHIFP EGSEPKVYLL YRPGHYDILY K |
Otubain 1 (OTUB1) belongs to the ovarian tumor (OUT) superfamily of predicted cysteine proteases and inhibits cytokine gene transcription in the immune system through its interaction with a ubiquitin protease and E3 ubiquitin ligase. OTUB1 is a highly specific ubiquitin iso-peptidase, it cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. OTUB1 is believed to work in specific ubiquitin-dependent pathways, possibly by providing an editing function of polyubiquitin chain growth. OTUB1 is a hydrolase that removes conjugated ubiquitin from proteins in vitro and may therefore have a significant regulatory role in the level of protein turnover by preventing degradation. Additionally, OTUB1 is a regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered impassive to antigen re-challenge and no longer respond to their cognate antigen. OTUB1 acts via its interaction with RNF128/GRAIL, which is an essential inductor of CD4 T-cell anergy.
OTUB1 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 291 amino acids (1- 271 a.a.) and having a molecular mass of 33.4kDa.The OTUB1 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | OTUB1: a protease that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occur when T-cell are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Two alternatively spliced isoforms are known. |
| UniProt Protein Details: | Protein type:Ubiquitin-specific protease; EC 3.4.19.12; Protease; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 11q13.1 Cellular Component: cytoplasm; nucleus Molecular Function:NEDD8-specific protease activity; protein binding; ubiquitin binding; ubiquitin protein ligase binding; ubiquitin-specific protease activity Biological Process: adaptive immune response; DNA repair; response to DNA damage stimulus |
| NCBI Summary: | The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q96FW1 |
| NCBI GenInfo Identifier: | 44888286 |
| NCBI Gene ID: | 55611 |
| NCBI Accession: | Q96FW1.2 |
| UniProt Secondary Accession: | Q96FW1,Q32Q78, Q96II3, Q9NXQ4, Q9P0B8, |
| UniProt Related Accession: | Q96FW1 |
| Molecular Weight: | 35,285 Da |
| NCBI Full Name: | Ubiquitin thioesterase OTUB1 |
| NCBI Synonym Full Names: | OTU deubiquitinase, ubiquitin aldehyde binding 1 |
| NCBI Official Symbol: | OTUB1�� |
| NCBI Official Synonym Symbols: | OTB1; OTU1; HSPC263�� |
| NCBI Protein Information: | ubiquitin thioesterase OTUB1 |
| UniProt Protein Name: | Ubiquitin thioesterase OTUB1 |
| UniProt Synonym Protein Names: | Deubiquitinating enzyme OTUB1; OTU domain-containing ubiquitin aldehyde-binding protein 1; Otubain-1; hOTU1; Ubiquitin-specific-processing protease OTUB1 |
| Protein Family: | Ubiquitin thioesterase |
| UniProt Gene Name: | OTUB1�� |
| UniProt Entry Name: | OTUB1_HUMAN |
