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Human Osteoclast Associated Receptor (OSCAR) ELISA Kit

SKU:
HUDL02147
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8IYS5
Sensitivity:
0.136ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
PIGR3,Osteoclast Associated,Immunoglobulin-Like Receptor,Polymeric immunoglobulin receptor 3,Poly-Ig receptor 3
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Osteoclast Associated Receptor (OSCAR) ELISA Kit

The Human Osteoclast-Associated Receptor (OSCAR) ELISA Kit is a comprehensive tool for the accurate measurement of OSCAR levels in human serum, plasma, and cell culture supernatants. This kit is designed with a focus on high sensitivity and specificity, guaranteeing precise and consistent results for a variety of research applications. OSCAR is a key receptor involved in bone metabolism, specifically in regulating osteoclast function and differentiation.

Its role in bone resorption makes it a critical target for studying conditions such as osteoporosis, arthritis, and other bone-related disorders. By accurately measuring OSCAR levels, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially identify new therapeutic strategies. Invest in the Human OSCAR ELISA Kit for reliable and reproducible data, advancing your research in bone biology and related fields.

Product Name:Human Osteoclast Associated Receptor (OSCAR) ELISA Kit
Product Code:HUDL02147
Size:96 Assays
Target:OSCAR
Synonyms:PIGR3, Osteoclast Associated,Immunoglobulin-Like Receptor, Polymeric immunoglobulin receptor 3, Poly-Ig receptor 3
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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