The Human OSM (Oncostatin M) CLIA Kit is specifically designed for the precise measurement of Oncostatin M levels in human samples including serum, plasma, and cell culture supernatants. This cutting-edge kit offers exceptional sensitivity and selectivity, ensuring dependable and reproducible results for a variety of research applications.Oncostatin M is a key cytokine that plays a crucial role in inflammatory responses, immune regulation, and tissue repair. Dysregulation of Oncostatin M has been linked to various diseases such as cancer, autoimmune disorders, and inflammatory conditions, making it a valuable biomarker for understanding disease mechanisms and developing potential treatments.
Overall, the Human OSM CLIA Kit from Assaygenie provides researchers with a powerful tool to accurately quantify Oncostatin M levels in human samples, thus enabling insights into the role of this cytokine in health and disease.
Product Name:
Human OSM (Oncostatin M) CLIA Kit
SKU:
HUES01178
Target:
Human OSM (Oncostatin M)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human OSM. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human OSM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human OSM, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human OSM. You can calculate the concentration of Human OSM in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-103
96-113
93-104
Average (%)
96
104
99
1:4
Range (%)
88-100
88-103
94-107
Average (%)
93
94
101
1:8
Range (%)
90-104
87-99
92-105
Average (%)
98
93
98
1:16
Range (%)
91-103
85-99
98-110
Average (%)
97
91
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-110
103
EDTA plasma (n=5)
91-102
97
Cell culture media (n=5)
95-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.21
54.57
204.27
28.18
50.28
205.66
Standard deviation
3.22
4.22
18.0
2.86
4.87
12.36
C V (%)
11.83
7.73
8.81
10.15
9.69
6.01
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human OSM concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human OSM in samples. No significant cross-reactivity or interference between Human OSM and analogues was observed.
