The Human ORM2 (Orosomucoid 2) ELISA Kit is developed for the precise quantitative measurement of Orosomucoid 2 levels in diverse human biological samples. Orosomucoid 2, also known as alpha-1-acid glycoprotein, is an acute-phase protein involved in acute inflammatory and immune responses.
Accurate detection of ORM2 is crucial for understanding its role in inflammation, infection, and various pathophysiological conditions, making it a valuable biomarker for research and clinical investigations. Our ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results in the quantification of ORM2. Manufactured under rigorous quality control standards, this kit guarantees robust performance and ease of use, making it an ideal choice for a wide range of applications. Trust Assay Genie's Human ORM2 ELISA Kit for reliable and precise measurements of this significant protein in your studies.
Product Name:
Human ORM2 (Orosomucoid 2) ELISA Kit
SKU:
AEES00122
Target:
Human ORM2 (Orosomucoid 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ORM2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ORM2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ORM2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ORM2. You can calculate the concentration of Human ORM2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-105
97-111
87-103
Average (%)
99
103
95
1:4
Range (%)
87-102
94-107
97-110
Average (%)
94
101
105
1:8
Range (%)
92-98
90-103
86-93
Average (%)
95
95
90
1:16
Range (%)
93-106
88-97
90-106
Average (%)
100
91
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-108
100
EDTA plasma (n=5)
94-106
102
Cell culture media (n=5)
82-96
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.82
2.36
8.39
0.82
2.33
9.4
Standard deviation
0.04
0.11
0.36
0.04
0.11
0.45
C V (%)
4.88
4.66
4.29
4.88
4.72
4.79
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ORM2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ORM2 in samples. No significant cross-reactivity or interference between Human ORM2 and analogues was observed.
