The Human Osteonectin CLIA Kit (HUES00787) is a state-of-the-art assay designed for the accurate measurement of osteonectin levels in human biological samples. This kit offers high sensitivity and specificity, providing researchers with reliable and reproducible results for a variety of research applications.Osteonectin, also known as SPARC (Secreted Protein Acidic and Rich in Cysteine), is a key protein involved in bone formation and remodeling. It also plays a role in various biological processes, including cell adhesion, migration, and angiogenesis.
Abnormal levels of osteonectin have been linked to diseases such as osteoporosis, cancer, and cardiovascular disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.The Human Osteonectin CLIA Kit (HUES00787) is an essential tool for researchers seeking to explore the role of osteonectin in health and disease. With its user-friendly format and accurate results, this kit is ideal for a wide range of research studies and holds great promise for advancing our understanding of osteonectin biology.
Product Name:
Human ON (Osteonectin) CLIA Kit
SKU:
HUES00787
Target:
Human ON (Osteonectin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ON. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ON and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ON, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ON. You can calculate the concentration of Human ON in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-118
94-106
86-102
Average (%)
109
99
93
1:4
Range (%)
91-107
95-109
96-111
Average (%)
98
100
103
1:8
Range (%)
92-107
95-111
87-98
Average (%)
99
102
93
1:16
Range (%)
93-107
101-117
90-105
Average (%)
101
107
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
95
EDTA plasma (n=5)
97-110
104
Cell culture media (n=5)
93-108
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
199.57
606.36
1519.01
187.2
654.78
1437.17
Standard deviation
23.89
61.97
136.86
17.73
62.2
99.45
C V (%)
11.97
10.22
9.01
9.47
9.5
6.92
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ON concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ON in samples. No significant cross-reactivity or interference between Human ON and analogues was observed.
