The Human OC (Osteocalcin) CLIA Kit is specifically designed for the quantitative measurement of Osteocalcin levels in human serum, plasma, and cell culture supernatants. With a high level of sensitivity and specificity, this kit ensures accurate and reproducible results for a variety of research applications.Osteocalcin, also known as Bone Gla Protein (BGP), is a key marker of bone metabolism and is involved in the regulation of bone formation and mineralization. Monitoring Osteocalcin levels can provide valuable insights into conditions such as osteoporosis, bone fractures, and metabolic disorders affecting bone health.
This kit is an essential tool for researchers studying bone biology, osteoporosis, and other bone-related diseases, as well as clinicians monitoring bone health in patients. With its reliable performance and easy-to-use format, the Human OC (Osteocalcin) CLIA Kit is a valuable addition to any laboratory conducting research in the field of bone biology.
Product Name:
Human OC/BGP (Osteocalcin) CLIA Kit
SKU:
HUES00779
Target:
Human OC/BGP (Osteocalcin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human OC/BGP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human OC/BGP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human OC/BGP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human OC/BGP. You can calculate the concentration of Human OC/BGP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
101-117
91-104
Average (%)
96
107
96
1:4
Range (%)
93-105
92-110
83-95
Average (%)
99
100
90
1:8
Range (%)
87-102
99-113
100-115
Average (%)
94
105
107
1:16
Range (%)
89-103
88-100
92-104
Average (%)
96
93
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-108
99
EDTA plasma (n=5)
101-115
107
Cell culture media (n=5)
87-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.91
2.85
8.86
1.0
3.06
9.5
Standard deviation
0.08
0.29
0.88
0.11
0.34
0.77
C V (%)
8.79
10.18
9.93
11.0
11.11
8.11
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human OC/BGP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human OC/BGP in samples. No significant cross-reactivity or interference between Human OC/BGP and analogues was observed.
