Human NUDT5 Recombinant Protein (RPPB2052)
- SKU:
- RPPB2052
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9UKK9
- Research Area:
- Enzymes
Description
| Product Name: | Human NUDT5 Recombinant Protein |
| Product Code: | RPPB2052 |
| Size: | 20µg |
| Species: | Human |
| Target: | NUDT5 |
| Synonyms: | hYSAH1, YSA1, YSA1H, ADP-sugar pyrophosphatase, EC=3.6.1.-, EC=3.6.1.13, Nucleoside diphosphate-linked moiety X motif 5, Nudix motif 5, HSPC115. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | NUDT5 Human 1mg/ml solution containing 20mM Tris-HCl pH-8, 1mM DTT, 0.1M NaCl & 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MESQEPTESS QNGKQYIISE ELISEGKWVK LEKTTYMDPT GKTRTWESVK RTTRKEQTAD GVAVIPVLQR TLHYECIVLV KQFRPPMGGY CIEFPAGLID DGETPEAAAL RELEEETGYK GDIAECSPAV CMDPGLSNCT IHIVTVTING DDAENARPKP KPGDGEFVEV ISLPKNDLLQ RLDALVAEEH LTVDARVYSY ALALKHANAK PFEVPFLKF |
NUDT5 is part of the nudix hydrolase family which remove toxic nucleotide derivatives from the cell. NUDT5 hydrolyzes ADP-ribose and ADP-mannose in the presence of magnesium, and in addition hydrolyzes nucleotide sugars with decreasing activity such as ADP-glucose and diadenosine diphosphate. As a nudix hydrolase, NUDT5 holds a central nudix motif and functions to eliminate toxic nucleotide metabolites from the cell while maintaining the levels of signaling nucleotides. NUDT5 is broadly expressed but is most abundant in liver as a homodimer.
NUDT5 Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 239 amino acids (1-219 a.a.) and having a molecular mass of 26.5 kDa. The NUDT5 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | NUDT5: Hydrolyzes with similar activities ADP-ribose ADP- mannose, ADP-glucose, 8-oxo-GDP and 8-oxo-dGDP. Can also hydrolyze other nucleotide sugars with low activity. Belongs to the Nudix hydrolase family. |
| UniProt Protein Details: | Protein type:EC 3.6.1.13; EC 3.6.1.58; Nucleotide Metabolism - purine; Phosphatase Chromosomal Location of Human Ortholog: 10p14 Cellular Component: cytosol; nucleus Molecular Function:ADP-ribose diphosphatase activity; ADP-sugar diphosphatase activity; m7G(5')pppN diphosphatase activity; magnesium ion binding; nucleotidyltransferase activity; protein binding; protein homodimerization activity; snoRNA binding Biological Process: chromatin remodeling; D-ribose catabolic process; ribonucleoside diphosphate catabolic process |
| NCBI Summary: | This gene belongs to the Nudix (nucleoside diphosphate linked moiety X) hydrolase superfamily. The encoded enzyme catalyzes the hydrolysis of modified nucleoside diphosphates, including ADP-ribose (ADPR) and 8-oxoGua-containing 8-oxo-dADP and 8-oxo-dGDP. Protein-bound ADP ribose can be hazardous to the cell because it can modify some amino acid residues, resulting in the inhibition of ATP-activated potassium channels. 8-oxoGua is an oxidized form of guanine that can potentially alter genetic information by pairing with adenine and cytosine in RNA. Presence of 8-oxoGua in RNA results in formation of abnormal proteins due to translational errors. [provided by RefSeq, Aug 2013] |
| UniProt Code: | Q9UKK9 |
| NCBI GenInfo Identifier: | 20455192 |
| NCBI Gene ID: | 11164 |
| NCBI Accession: | Q9UKK9.1 |
| UniProt Secondary Accession: | Q9UKK9,Q6IAG0, Q9UH49, A8K516, |
| UniProt Related Accession: | Q9UKK9 |
| Molecular Weight: | 24kDa |
| NCBI Full Name: | ADP-sugar pyrophosphatase |
| NCBI Synonym Full Names: | nudix hydrolase 5 |
| NCBI Official Symbol: | NUDT5�� |
| NCBI Official Synonym Symbols: | YSA1; YSA1H; YSAH1; hNUDT5�� |
| NCBI Protein Information: | ADP-sugar pyrophosphatase |
| UniProt Protein Name: | ADP-sugar pyrophosphatase |
| UniProt Synonym Protein Names: | 8-oxo-dGDP phosphatase (EC:3.6.1.58 |
| Protein Family: | Nudix hydrolase |
| UniProt Gene Name: | NUDT5�� |
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