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Human Nuclear Transcription Factor Y Gamma (NFYC) ELISA Kit

SKU:
HUDL02030
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q13952
Sensitivity:
0.114ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
CBF-C,NFYg,CBFC,H1TF2A,HAP5,HSM,NF-YC,hCBF-C,CAAT box DNA-binding protein subunit C,Nuclear transcription factor Y subunit C,Transactivator HSM-1/2
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

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Human Nuclear Transcription Factor Y Gamma (NFYC) ELISA Kit

The Human Nuclear Transcription Factor Y Gamma (NFYC) ELISA Kit is specifically designed to accurately detect levels of NFYC in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it perfect for a variety of research applications.NFYC is a key transcription factor that plays a crucial role in regulating gene expression and controlling various cellular processes. It is involved in numerous biological pathways, including cell growth, differentiation, and apoptosis.

Dysregulation of NFYC has been linked to various diseases, making it a valuable biomarker for studying disease mechanisms and potential therapeutic interventions.Whether you are studying cancer, neurological disorders, or other conditions impacted by NFYC, this ELISA kit provides a powerful tool for accurate quantification and analysis. Trust in the precision and reliability of the Human Nuclear Transcription Factor Y Gamma (NFYC) ELISA Kit for your research needs.

Product Name:Human Nuclear Transcription Factor Y Gamma (NFYC) ELISA Kit
Product Code:HUDL02030
Size:96 Assays
Target:NFYC
Synonyms:CBF-C, NFYg, CBFC, H1TF2A, HAP5, HSM, NF-YC, hCBF-C, CAAT box DNA-binding protein subunit C, Nuclear transcription factor Y subunit C, Transactivator HSM-1/2
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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