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Human Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1) ELISA Kit

SKU:
HUDL02086
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P04150
Sensitivity:
0.064ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
GR,GCCR,GCR,GR,GRL,NR3-C1,Glucocorticoid Receptor
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

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Human Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1) ELISA Kit

The Human Nuclear Receptor Subfamily 3 Group C Member 1 (NR3C1) ELISA Kit is a highly sensitive and specific assay designed for the accurate measurement of NR3C1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.NR3C1, also known as the glucocorticoid receptor, is a key protein involved in the regulation of gene expression in response to glucocorticoid hormones. Dysregulation of NR3C1 has been implicated in various diseases, including inflammatory disorders, metabolic conditions, and psychiatric disorders.

Therefore, measuring NR3C1 levels can provide valuable insights into disease pathology and potential therapeutic targets.By using the Human NR3C1 ELISA Kit, researchers can accurately quantify NR3C1 levels in human samples, allowing for a better understanding of its role in disease processes and potential treatment strategies. Trust in the precision and reliability of this kit for your research needs.

Product Name:Human Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1) ELISA Kit
Product Code:HUDL02086
Size:96 Assays
Target:NR3C1
Synonyms:GR, GCCR, GCR, GR, GRL, NR3-C1, Glucocorticoid Receptor
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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