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Human Nuclear Receptor Coactivator 3 (NCOA3) ELISA Kit

SKU:
HUDL01998
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9Y6Q9
Sensitivity:
0.117ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
RAC3,ACTR,AIB1,CAGH16,CTG26,SRC3,TNRC14,TNRC16,TRAM1,pCIP,Amplified In Breast 1,Steroid Receptor Coactivator-3,Thyroid Hormone Receptor Activator Molecule 1
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Nuclear Receptor Coactivator 3 (NCOA3) ELISA Kit

The Human Nuclear Receptor Coactivator 3 (NCOA3) ELISA Kit is a reliable and accurate tool for the detection of NCOA3 levels in human samples such as serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides dependable and consistent results for a variety of research applications.NCOA3 is a critical component in gene transcription, mediating the activation of nuclear receptors and playing a key role in various physiological processes.

Dysregulation of NCOA3 has been linked to diseases such as cancer, metabolic disorders, and hormonal imbalances, making it a valuable biomarker for investigating these conditions and exploring potential therapeutic interventions.Overall, the Human NCOA3 ELISA Kit offers researchers a powerful tool for studying the role of NCOA3 in disease pathology and identifying novel targets for intervention.

Product Name:Human Nuclear Receptor Coactivator 3 (NCOA3) ELISA Kit
Product Code:HUDL01998
Size:96 Assays
Target:NCOA3
Synonyms:RAC3, ACTR, AIB1, CAGH16, CTG26, SRC3, TNRC14, TNRC16, TRAM1, pCIP, Amplified In Breast 1, Steroid Receptor Coactivator-3, Thyroid Hormone Receptor Activator Molecule 1
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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