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Human Nuclear Factor Kappa B (NFkB) ELISA Kit

SKU:
HUDL02028
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P19838
Sensitivity:
0.063ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
NF-KB1,EBP-1,KBF1,NF-Kappa-B,NFKB-P105,NFKB-P50,Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1,Nuclear factor NF-kappa-B p105 subunit
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

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Human Nuclear Factor Kappa B (NFkB) ELISA Kit

The Human Nuclear Factor Kappa B (NFkB) ELISA Kit is specifically designed for the precise quantification of NFkB levels in human cell lysates, tissue homogenates, and other biological samples. With its exceptional sensitivity and specificity, this kit delivers accurate and reproducible results, making it an indispensable tool for a variety of research applications.NFkB is a key transcription factor that regulates a wide range of genes involved in inflammation, immunity, and cell survival. Dysregulation of NFkB signaling has been implicated in various diseases, including cancer, inflammatory disorders, and autoimmune conditions.

Therefore, measuring NFkB levels can provide valuable insights into disease pathogenesis and potential therapeutic targets.With the Human NFkB ELISA Kit, researchers can gain valuable information about NFkB activity in different biological samples, enabling a better understanding of disease mechanisms and the development of novel treatment strategies. Elevate your research with this cutting-edge ELISA kit from Assay Genie.

Product Name:Human Nuclear Factor Kappa B (NFkB) ELISA Kit
Product Code:HUDL02028
Size:96 Assays
Target:NFkB
Synonyms:NF-KB1, EBP-1, KBF1, NF-Kappa-B, NFKB-P105, NFKB-P50, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1, Nuclear factor NF-kappa-B p105 subunit
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)87-10093
EDTA plasma (n=5)89-9994
Heparin plasma (n=5)90-9894
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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