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Human Nuclear Factor I/B (NFIB) ELISA Kit

SKU:
HUDL02025
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O00712
Sensitivity:
0.054ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
NFI-RED,NFIB2,NFIB3,CCAAT-box-binding transcription factor,TGGCA-binding protein
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Nuclear Factor I/B (NFIB) ELISA Kit

The Human Nuclear Factor I-B (NFIB) ELISA Kit is a reliable and sensitive assay designed for the precise quantification of NFIB levels in human samples including serum, plasma, and cell culture supernatants. This kit offers high specificity and accuracy, delivering reproducible results for a variety of research applications.NFIB is a key transcription factor that plays a crucial role in regulating gene expression and cellular processes. Dysregulation of NFIB has been implicated in various diseases including cancer, developmental disorders, and neurological conditions, making it a valuable biomarker for understanding disease mechanisms and developing targeted therapies.

With its advanced technology and proven performance, the Human NFIB ELISA Kit is an essential tool for researchers studying the role of NFIB in health and disease, providing valuable insights into molecular pathways and potential therapeutic targets.

Product Name:Human Nuclear Factor I/B (NFIB) ELISA Kit
Product Code:HUDL02025
Size:96 Assays
Target:NFIB
Synonyms:NFI-RED, NFIB2, NFIB3, CCAAT-box-binding transcription factor, TGGCA-binding protein
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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