Human NT-proBNP (N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
The Human NT-proBNP (N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit is meticulously crafted to enable the precise quantitative detection of N-Terminal Pro-Brain Natriuretic Peptide in a variety of human biological samples.
This biomarker serves as a critical indicator of cardiac function and is essential for assessing heart failure and related cardiovascular conditions. NT-proBNP plays a significant role in regulating blood pressure, fluid balance, and overall cardiac health. Our ELISA kit boasts exceptional sensitivity and specificity, ensuring accurate and reproducible results vital for studying cardiovascular diseases and monitoring cardiac function. Manufactuctured under stringent quality control standards, this kit delivers reliable and robust performance, combined with user-friendly protocols, making it an exceptional choice for research applications. Count on Assay Genie's NT-proBNP ELISA Kit for precise and dependable quantification of this pivotal cardiac biomarker in your research endeavors.
Product Name:
Human NT-proBNP (N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
SKU:
AEES00154
Target:
Human NT-proBNP (N-Terminal Pro-Brain Natriuretic Peptide)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NT-proBNP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NT-proBNP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NT-proBNP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NT-proBNP. You can calculate the concentration of Human NT-proBNP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
94-110
85-98
Average (%)
91
101
91
1:4
Range (%)
85-98
94-107
85-99
Average (%)
92
101
92
1:8
Range (%)
85-97
93-107
83-96
Average (%)
91
98
90
1:16
Range (%)
86-100
94-107
88-99
Average (%)
92
101
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-107
98
EDTA plasma (n=5)
92-102
97
Cell culture media (n=5)
88-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.53
1.27
3.97
0.57
1.39
3.95
Standard deviation
0.03
0.06
0.22
0.03
0.07
0.17
C V (%)
6.45
4.45
5.47
5.06
4.85
4.28
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human NT-proBNP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NT-proBNP in samples. No significant cross-reactivity or interference between Human NT-proBNP and analogues was observed.
