Human NT-proANP (N-Terminal Pro Atrial Natriuretic Peptide) ELISA Kit
The Human NT-proANP (N-Terminal Pro Atrial Natriuretic Peptide) ELISA Kit is meticulously designed for the quantitative measurement of N-Terminal Pro Atrial Natriuretic Peptide in various human biological samples. NT-proANP, a precursor of atrial natriuretic peptide, serves as a biomarker for cardiac function and volume status, playing a key role in regulating blood pressure and fluid balance. Accurate quantification of NT-proANP is crucial in cardiovascular research and clinical diagnosis, offering insights into cardiac health and related conditions.
With exceptional sensitivity and specificity, Assay Genie's NT-proANP ELISA Kit ensures precise and reproducible results, making it a reliable tool for biomarker analysis. Manufactured under strict quality assurance measures, this kit delivers robust performance and user-friendly protocols suitable for diverse research and clinical applications. Count on Assay Genie's NT-proANP ELISA Kit for accurate and trustworthy quantification of this significant cardiac biomarker in your scientific endeavors.
Product Name:
Human NT-proANP (N-Terminal Pro Atrial Natriuretic Peptide) ELISA Kit
SKU:
AEES00124
Target:
Human NT-proANP (N-Terminal Pro Atrial Natriuretic Peptide)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NT-proANP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NT-proANP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NT-proANP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NT-proANP. You can calculate the concentration of Human NT-proANP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
89-99
91-104
Average (%)
90
92
97
1:4
Range (%)
95-109
96-109
91-104
Average (%)
102
101
99
1:8
Range (%)
91-106
89-102
94-102
Average (%)
99
96
98
1:16
Range (%)
89-102
96-106
91-105
Average (%)
97
100
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
83-96
91
EDTA plasma (n=5)
87-97
91
Cell culture media (n=5)
99-110
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.71
4.66
17.41
1.69
4.28
16.39
Standard deviation
0.09
0.21
0.87
0.09
0.19
0.76
C V (%)
5.26
4.51
5.0
5.33
4.44
4.64
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human NT-proANP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NT-proANP in samples. No significant cross-reactivity or interference between Human NT-proANP and analogues was observed.
