The Human NT (Neurotensin) ELISA Kit is specifically designed for the precise measurement of neurotensin levels in human samples such as serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring accurate and consistent results for various research applications.Neurotensin is a neuropeptide that acts as a neurotransmitter and hormone in the body, playing a critical role in regulating various physiological processes such as pain perception, stress response, and gastrointestinal function. Dysregulation of neurotensin levels has been linked to conditions like schizophrenia, Parkinson's disease, and inflammatory bowel disease, making it a valuable biomarker for studying these disorders and potentially developing novel therapeutic approaches.
With the Human NT (Neurotensin) ELISA Kit, researchers can easily quantify neurotensin levels in human samples, allowing for deeper insights into the role of this neuropeptide in health and disease. Trust in the reliability and precision of this kit to advance your research efforts in the field of neurobiology and beyond.
Product Name:
Human NT (Neurotensin) ELISA Kit
SKU:
HUES02835
Target:
Human NT (Neurotensin)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with NT. During the reaction, NT in the sample or standard competes with a fixed amount of NT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to NT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NT in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-103
97-115
91-105
Average (%)
94
105
96
1:4
Range (%)
91-105
88-100
92-106
Average (%)
96
95
100
1:8
Range (%)
90-104
92-102
96-112
Average (%)
97
97
104
1:16
Range (%)
89-100
91-105
93-109
Average (%)
95
97
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-99
90
EDTA plasma (n=5)
90-104
97
Cell culture media (n=5)
91-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
227.6
449.5
2334.7
207.4
442.4
2410.5
Standard deviation
12.7
26.1
109.7
12.2
23.9
118.1
C V (%)
5.58
5.81
4.7
5.88
5.4
4.9
Sample type &Sample volume:
Serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of NT concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes NT in samples. No significant cross-reactivity or interference between NT and analogues was observed.
