Based on the information provided at the URL, here is a draft product description:The Human NRG-2 (Neuregulin-2) CLIA Kit is a cutting-edge assay specifically designed for the precise measurement of Neuregulin-2 levels in human samples such as serum, plasma, and cell culture supernatants. This innovative kit offers unparalleled sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Neuregulin-2 is a critical signaling molecule that plays a crucial role in various physiological processes, including nerve development, cardiac function, and synaptic plasticity. Dysregulation of Neuregulin-2 levels has been linked to various diseases such as schizophrenia, cancer, and heart disorders, highlighting its significance as a biomarker for disease research and therapeutic development.
Incorporating advanced technology and rigorous quality control measures, the Human NRG-2 CLIA Kit from Assay Genie provides researchers with a reliable tool to investigate the role of Neuregulin-2 in health and disease. Whether studying molecular pathways or exploring potential treatment strategies, this kit offers a comprehensive solution for researchers seeking to unravel the complexities of Neuregulin-2 biology.
Product Name:
Human NRG-2 (Neuregulin 2) CLIA Kit
SKU:
HUES00539
Target:
Human NRG-2 (Neuregulin 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NRG-2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NRG-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NRG-2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NRG-2. You can calculate the concentration of Human NRG-2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-110
101-116
95-108
Average (%)
100
107
100
1:4
Range (%)
98-117
87-100
94-108
Average (%)
107
93
99
1:8
Range (%)
100-118
99-114
94-108
Average (%)
108
105
99
1:16
Range (%)
96-109
87-102
101-116
Average (%)
102
93
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
91
EDTA plasma (n=5)
97-110
104
Cell culture media (n=5)
91-104
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.32
176.07
901.79
107.59
174.5
914.38
Standard deviation
11.32
19.37
82.24
9.63
12.79
69.22
C V (%)
10.75
11.0
9.12
8.95
7.33
7.57
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human NRG-2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NRG-2 in samples. No significant cross-reactivity or interference between Human NRG-2 and analogues was observed.
