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Human NPPA Recombinant Protein (RPPB0809)

SKU:
RPPB0809
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P01160
Research Area:
Cytokines
$371
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Description

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Product Name:Human NPPA Recombinant Protein
Product Code:RPPB0809
Size:10µg
Species:Human
Target:NPPA
Synonyms:Natriuretic peptides A, CDD-ANF, Cardiodilatin, CDD, Cardiodilatin-related peptide, CDP, N-terminal proatrial natriuretic peptide, ANP, PND.�
Source:Escherichia Coli
Physical Appearance:Filtered White lyophilized (freeze-dried) powder.
Formulation:NPPA filtered (0.4 �m) and lyophilized from 0.5mg/ml in 20 mM Tris buffer, 50 mM NaCl and 5% w/v trehalosa, pH 7.5.
Solubility:It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. NPPA is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Stability:Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MKHHHHHHNP MYNAVSNADL MDFKNLLDHL EEKMPLEDEV VPPQVLSEPN EEAGAALSPL PEVPPWTGEV SPAQRDGGAL GRGPWDSSDR SALLKSKLRA LLTAPR

Natriuretic Peptide A (NPPA) is a part of the natriuretic peptide family and is involved �in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. NPPA is synthesized as a great precursor which releases a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. In female pregnancy, NPPA is promoting trophoblast invasion and spiral artery remodeling in uterus. �

NPPA Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (a.a 26-123) containing 106 amino acids including an 8 a.a N-terminal His tag. The total molecular mass is 11.7kDa (calculated).�

UniProt Protein Function:NPPA: Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. Defects in NPPA are the cause of familial atrial fibrillation type 6 (ATFB6). Atrial fibrillation is a common disorder of cardiac rhythm that is hereditary in a small subgroup of patients. It is characterized by disorganized atrial electrical activity, progressive deterioration of atrial electromechanical function and ineffective pumping of blood into the ventricles. It can be associated with palpitations, syncope, thromboembolic stroke, and congestive heart failure. Belongs to the natriuretic peptide family.
UniProt Protein Details:

Protein type:Hormone; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p36.21

Cellular Component: extracellular space; mast cell granule; perinuclear region of cytoplasm; extracellular region; nucleus

Molecular Function:neuropeptide hormone activity; peptide hormone receptor binding; receptor binding

Biological Process: cardiac muscle hypertrophy; transcription initiation from RNA polymerase II promoter; cGMP biosynthetic process; negative regulation of systemic arterial blood pressure; positive regulation of heart rate; female pregnancy; response to insulin stimulus; neuropeptide signaling pathway; receptor guanylyl cyclase signaling pathway; regulation of blood pressure; response to hypoxia; gene expression; negative regulation of cell growth; regulation of blood vessel size

Disease: Atrial Standstill 2; Atrial Fibrillation, Familial, 6

NCBI Summary:The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. [provided by RefSeq, Sep 2009]
UniProt Code:P01160
NCBI GenInfo Identifier:113864
NCBI Gene ID:4878
NCBI Accession:P01160.1
UniProt Secondary Accession:P01160,Q13766, Q5JZE1,
UniProt Related Accession:P01160
Molecular Weight:153
NCBI Full Name:Natriuretic peptides A
NCBI Synonym Full Names:natriuretic peptide A
NCBI Official Symbol:NPPA��
NCBI Official Synonym Symbols:ANF; ANP; PND; ATFB6; ATRST2; CDD-ANF��
NCBI Protein Information:natriuretic peptides A; atriopeptin; cardionatrin; prepronatriodilatin; natriuretic peptide precursor A
UniProt Protein Name:Natriuretic peptides A
UniProt Synonym Protein Names:CDD-ANF; PrepronatriodilatinCleaved into the following 2 chains:Cardiodilatin-related peptide; CDP; Atrial natriuretic factor; ANFAlternative name(s):Atrial natriuretic peptide; ANP
UniProt Gene Name:NPPA��
UniProt Entry Name:ANF_HUMAN

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