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Human NPM1 Recombinant Protein (RPPB4119)

SKU:
RPPB4119
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P06748
€309
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Description

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Product Name:Human NPM1 Recombinant Protein
Product Code:RPPB4119
Size:20µg
Species:Human
Target:NPM1
Synonyms:Nucleophosmin, NPM, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin, NPM1, NPM, B23.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:NPM1 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.15M NaCl and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MEDSMDMDMS PLRPQNYLFG CELKADKDYH FKVDNDENEH QLSLRTVSLG AGAKDELHIV EAEAMNYEGS PIKVTLATLK MSVQPTVSLG GFEITPPVVL RLKCGSGPVH ISGQHLVAVE EDAESEDEEE EDVKLLSISG KRSAPGGGSK VPQKKVKLAADEDDDDDDEE DDDEDDDDDD FDDEEAEEKA PVKKSIRDTP AKNAQKSNQN GKDSKPSSTP RSKGQESFKK QEKTPKTPKG PSSVEDIKAK MQASIEKGGS LPKVEAKFIN YVKNCFRMTD QEAIQDLWQW RKSL

Nucleophosmin (NPM1) is a phosphoprotein which shuffles between the nucleus and the cytoplasm. NPM1 is assumed to be involved in a number of processes including regulation of the ARF/p53 pathway. Several genes fusion partners have been described, particularly the anaplastic lymphoma kinase gene on chromosome 2. NPM1 gene mutations are linked with acute myeloid leukemia.

NPM1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 314 amino acids (1-294 a.a) and having a molecular mass of 34.7kDa.NPM1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:NPM1: a nucleolar protein associated with nucleolar ribonucleoprotein structures and that binds single-stranded nucleic acids. Is a major component of template activating factor (TAF)-III. It plays a role in controlling centrosome duplication, and may function in the assembly and/or transport of the ribosome. Two alternatively spliced isoforms have been described.
UniProt Protein Details:

Protein type:Translation; RNA-binding; Nucleolus; Oncoprotein

Chromosomal Location of Human Ortholog: 5q35.1

Cellular Component: centrosome; cytoplasm; cytosol; focal adhesion; membrane; nucleolus; nucleoplasm; nucleus; ribonucleoprotein complex; spindle pole centrosome

Molecular Function:histone binding; NF-kappaB binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding; protein kinase inhibitor activity; ribosomal large subunit binding; ribosomal small subunit binding; RNA binding; Tat protein binding; transcription coactivator activity; unfolded protein binding

Biological Process: activation of NF-kappaB transcription factor; cell aging; centrosome cycle; DNA repair; DNA replication-independent nucleosome assembly at centromere; intracellular protein transport; negative regulation of apoptosis; negative regulation of cell proliferation; nucleocytoplasmic transport; nucleosome assembly; positive regulation of cell proliferation; positive regulation of transcription, DNA-dependent; positive regulation of translation; protein localization; protein oligomerization; regulation of centriole replication; regulation of endodeoxyribonuclease activity; response to stress; ribosome assembly; signal transduction; viral reproduction

NCBI Summary:This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Nov 2009]
UniProt Code:P06748
NCBI GenInfo Identifier:114762
NCBI Gene ID:4869
NCBI Accession:P06748.2
UniProt Secondary Accession:P06748,P08693, Q12826, Q13440, Q13441, Q14115, Q5EU94 Q5EU95, Q5EU96, A8K3N7, B5BU00, D3DQL6,
UniProt Related Accession:P06748
Molecular Weight:28,400 Da
NCBI Full Name:Nucleophosmin
NCBI Synonym Full Names:nucleophosmin (nucleolar phosphoprotein B23, numatrin)
NCBI Official Symbol:NPM1��
NCBI Official Synonym Symbols:B23; NPM��
NCBI Protein Information:nucleophosmin
UniProt Protein Name:Nucleophosmin
UniProt Synonym Protein Names:Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin
Protein Family:Nucleophosmin
UniProt Gene Name:NPM1��
UniProt Entry Name:NPM_HUMAN

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