The Human NPHN (Nephrin) CLIA Kit is specifically designed for the precise measurement of NPHN levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it an invaluable tool for various research applications.Nephrin is a key protein involved in maintaining the integrity of the kidney filtration barrier, playing a crucial role in kidney function and health. Changes in Nephrin expression levels have been linked to various kidney disorders, such as proteinuric kidney diseases, diabetic nephropathy, and glomerular disorders.
Therefore, the Human NPHN CLIA Kit is essential for studying these conditions and identifying potential therapeutic targets.Overall, the Human NPHN (Nephrin) CLIA Kit is a reliable and effective solution for researchers looking to investigate the role of Nephrin in kidney diseases and related conditions. Get accurate and consistent results with this advanced kit from AssayGenie.
Product Name:
Human NPHN (Nephrin) CLIA Kit
SKU:
HUES01049
Target:
Human NPHN (Nephrin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NPHN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NPHN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NPHN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NPHN. You can calculate the concentration of Human NPHN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-108
102-118
98-114
Average (%)
98
108
106
1:4
Range (%)
87-101
87-101
101-117
Average (%)
92
93
107
1:8
Range (%)
85-97
90-103
98-110
Average (%)
91
95
104
1:16
Range (%)
94-106
102-119
101-115
Average (%)
100
110
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-116
106
EDTA plasma (n=5)
87-98
93
Cell culture media (n=5)
91-102
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
90.91
218.23
958.24
95.89
213.02
1040.72
Standard deviation
9.9
20.6
81.55
12.36
17.98
97.62
C V (%)
10.89
9.44
8.51
12.89
8.44
9.38
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human NPHN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NPHN in samples. No significant cross-reactivity or interference between Human NPHN and analogues was observed.
