Human NOX5 (Nicotinamide Adenine Dinucleotide Phosphate Oxidase 5) ELISA Kit
The Human NOX5 (Nicotinamide Adenine Dinucleotide Phosphate Oxidase 5) ELISA Kit is a powerful tool for the precise detection of NOX5 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.NOX5 is a key enzyme involved in the production of reactive oxygen species, playing a crucial role in various physiological and pathological processes.
Its dysregulation has been linked to conditions such as cancer, cardiovascular diseases, and inflammatory disorders, highlighting its importance as a valuable biomarker for research and therapeutic development.With its reliable performance and ease of use, the Human NOX5 ELISA Kit is an indispensable resource for researchers studying the role of NOX5 in health and disease, providing valuable insights into potential treatment strategies and disease mechanisms.
Product Name:
Human NOX5 (Nicotinamide Adenine Dinucleotide Phosphate Oxidase 5) ELISA Kit
SKU:
HUES03115
Target:
Human NOX5 (Nicotinamide Adenine Dinucleotide Phosphate Oxidase 5)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NOX5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NOX5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NOX5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NOX5. You can calculate the concentration of Human NOX5 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
83-98
97-113
97-111
Average (%)
90
105
104
1:4
Range (%)
91-103
83-96
87-99
Average (%)
98
88
92
1:8
Range (%)
91-107
84-99
82-95
Average (%)
98
91
88
1:16
Range (%)
92-105
81-90
87-101
Average (%)
98
86
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
100
EDTA plasma (n=5)
88-101
93
Cell culture media (n=5)
87-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.89
220.44
938.13
97.64
229.99
959.29
Standard deviation
4.95
8.88
48.13
6.17
12.9
39.14
C V (%)
5.16
4.03
5.13
6.32
5.61
4.08
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human NOX5 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NOX5 in samples. No significant cross-reactivity or interference between Human NOX5 and analogues was observed.
