Human Notch2 N-Terminal Like Protein (NOTCH2NL) ELISA Kit
The Human Notch2 N-terminal Like Protein (Notch2NL) ELISA Kit is specifically designed for the precise measurement of Notch2NL levels in human serum, plasma, and cell culture supernatants. This kit is known for its high accuracy and specificity, ensuring dependable and consistent results, making it perfect for a variety of research purposes.Notch2NL is a protein that plays a key role in cellular signaling pathways and is involved in regulating various cellular processes. Studies have shown that abnormalities in Notch2NL levels are linked to several health conditions, including cancer, developmental disorders, and neurological diseases.
Therefore, monitoring Notch2NL levels can provide valuable insights into these conditions and aid in the development of potential treatments.Overall, the Human Notch2NL ELISA Kit is a valuable tool for researchers looking to investigate the role of Notch2NL in health and disease, offering reliable and accurate results for a wide range of applications.
Product Name:
Human Notch2 N-Terminal Like Protein (NOTCH2NL) ELISA Kit
Product Code:
HUDL02062
Size:
96 Assays
Target:
NOTCH2NL
Synonyms:
N2N
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
