Human NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal) ELISA Kit
The Human NOS1 (nNOS) Nitric Oxide Synthase 1 Neuronal ELISA Kit is a specialized assay designed for the precise measurement of NOS1 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results that are suitable for a wide range of research applications.NOS1, also known as neuronal nitric oxide synthase 1, is a key enzyme involved in the production of nitric oxide, a crucial signaling molecule in the body. Dysfunction of NOS1 has been implicated in various diseases such as cardiovascular disorders, neurodegenerative conditions, and inflammatory disorders.
Therefore, measuring NOS1 levels can provide valuable insights into the pathophysiology of these diseases and aid in the development of potential therapeutic strategies.By using the Human NOS1 (nNOS) Nitric Oxide Synthase 1 Neuronal ELISA Kit, researchers can accurately quantify NOS1 levels and further understand its role in disease progression. This kit is an indispensable tool for advancing research in the field of nitric oxide biology and exploring new therapeutic avenues for related disorders.
Product Name:
Human NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal) ELISA Kit
SKU:
HUES01887
Target:
Human NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NOS1/nNOS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NOS1/nNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NOS1/nNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NOS1/nNOS. You can calculate the concentration of Human NOS1/nNOS in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
93-107
93-106
Average (%)
100
99
99
1:4
Range (%)
100-113
87-100
91-107
Average (%)
107
92
98
1:8
Range (%)
98-112
84-98
98-114
Average (%)
106
90
104
1:16
Range (%)
100-114
83-94
94-105
Average (%)
107
89
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
92
EDTA plasma (n=5)
91-108
98
Cell culture media (n=5)
91-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.4
3.5
0.5
1.3
3.4
Standard deviation
0.03
0.07
0.19
0.03
0.05
0.16
C V (%)
6
5.0
5.43
6.0
3.85
4.71
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human NOS1/nNOS concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NOS1/nNOS in samples. No significant cross-reactivity or interference between Human NOS1/nNOS and analogues was observed.
