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Human Non POU Domain Containing Octamer Binding Protein (NONO) ELISA Kit

SKU:
HUDL02055
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q15233
Sensitivity:
0.126ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
P54,NMT55,NRB54,P54NRB,Nuclear RNA-Binding Protein 54-kD,54 kDa nuclear RNA- and DNA-binding protein,DNA-binding p52/p100 complex, 52 kDa subunit
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Non POU Domain Containing Octamer Binding Protein (NONO) ELISA Kit

The Human Non-POU Domain Containing Octamer-Binding Protein (NONO) ELISA Kit is a powerful tool for the accurate measurement of NONO levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.NONO is a key protein involved in various cellular processes, including gene expression regulation, RNA processing, and DNA repair.

Dysregulation of NONO has been linked to diseases such as cancer, neurodegenerative disorders, and autoimmune diseases, making it a valuable biomarker for studying disease mechanisms and potential therapeutic interventions.Overall, the Human NONO ELISA Kit is an essential resource for researchers investigating the role of NONO in health and disease, providing accurate and precise measurement of NONO levels in a convenient and user-friendly format.

Product Name:Human Non POU Domain Containing Octamer Binding Protein (NONO) ELISA Kit
Product Code:HUDL02055
Size:96 Assays
Target:NONO
Synonyms:P54, NMT55, NRB54, P54NRB, Nuclear RNA-Binding Protein 54-kD, 54 kDa nuclear RNA- and DNA-binding protein, DNA-binding p52/p100 complex, 52 kDa subunit
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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