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Human Non Metastatic Cells 1, Protein NM23A Expressed In (NME1) ELISA Kit

SKU:
HUDL02049
Product Type:
ELISA Kit
Sensitivity:
0.059ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
AWD,GAAD,NDPKA,NM23-H1,Nucleoside diphosphate kinase A,Granzyme A-activated DNase,Metastasis inhibition factor nm23,Tumor metastatic process-associated protein
Reactivity:
Human
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Non Metastatic Cells 1, Protein NM23A Expressed In (NME1) ELISA Kit

The Human NM23A (Non-Metastatic Cells 1 Protein) ELISA Kit is specifically designed for the accurate measurement of NM23A levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for various research applications.NM23A, also known as NME1, is a critical protein involved in cell metastasis regulation and tumor progression. It plays a crucial role in suppressing metastasis and has been implicated in various cancer types.

The detection of NM23A levels can provide valuable insights into the metastatic potential of cells and may serve as a potential biomarker for cancer diagnosis and prognosis.Overall, the Human NM23A ELISA Kit is a valuable tool for researchers studying cell metastasis, cancer biology, and potential therapeutic targets related to NM23A expression.

Product Name:Human Non Metastatic Cells 1, Protein NM23A Expressed In (NME1) ELISA Kit
Product Code:HUDL02049
Size:96 Assays
Target:NME1
Synonyms:AWD, GAAD, NDPKA, NM23-H1, Nucleoside diphosphate kinase A, Granzyme A-activated DNase, Metastasis inhibition factor nm23, Tumor metastatic process-associated protein
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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