The Human NLRP3 (NACHT, LRR, and PYD domains-containing protein 3) ELISA Kit is a state-of-the-art assay tailored for the precise quantitative analysis of NLRP3 protein levels in human samples.
NLRP3, a key component of the inflammasome complex, plays a crucial role in innate immunity regulation and inflammatory responses. Dysregulation of NLRP3 has been linked to various inflammatory conditions, autoimmune disorders, and infections, highlighting the significance of accurate measurement of NLRP3 levels in understanding immune system function and exploring therapeutic interventions. With exceptional sensitivity and specificity, the Human NLRP3 ELISA Kit enables researchers to quantitatively assess NLRP3 expression levels, facilitating investigations into the molecular mechanisms underlying inflammatory pathways and immune responses. By providing reliable data on NLRP3 protein concentrations, this ELISA kit empowers researchers to unravel the complexities of NLRP3-mediated inflammatory processes and immune system regulation. Manufactured under stringent quality control standards, the Human NLRP3 ELISA Kit ensures robust performance and reproducibility in quantifying NLRP3, offering a reliable tool for researchers studying inflammasome biology, immunology, and inflammatory diseases. Researchers can leverage this user-friendly kit to advance their investigations, gain insights into NLRP3-related mechanisms, and contribute to the development of targeted therapeutic strategies.
Product Name:
Human NLRP3 ELISA Kit
SKU:
AEES00672
Target:
Human NLRP3
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NLRP3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NLRP3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NLRP3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NLRP3. You can calculate the concentration of Human NLRP3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
97-114
94-106
Average (%)
92
105
100
1:4
Range (%)
85-99
97-111
90-102
Average (%)
92
103
97
1:8
Range (%)
89-101
101-117
90-103
Average (%)
94
107
96
1:16
Range (%)
83-97
101-115
92-105
Average (%)
89
107
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-96
91
EDTA plasma (n=5)
87-98
92
Cell culture media (n=5)
92-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.47
0.84
4.71
0.46
0.78
4.71
Standard deviation
0.03
0.04
0.17
0.03
0.04
0.16
C V (%)
6.38
4.76
3.61
6.52
5.13
3.4
Sample type &Sample volume:
; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human NLRP3 concentrations in .
Specificity:
This kit recognizes Human NLRP3 in samples. No significant cross-reactivity or interference between Human NLRP3 and analogues was observed.
