Human NFRkB (Nuclear Factor Related To Kappa B Binding Protein) CLIA Kit
The Human NFRKB (Nuclear Factor Related to Kappa-B Binding Protein) CLIA Kit is a highly reliable and sensitive assay designed for the accurate detection of NFRKB levels in human samples. This kit is ideal for studying the role of NFRKB in various biological processes such as transcriptional regulation, DNA repair, and apoptosis. NFRKB is a key protein involved in the regulation of gene expression and cellular processes, playing a critical role in immune response, inflammatory pathways, and development. Dysregulation of NFRKB has been associated with various diseases, including cancer, autoimmune disorders, and neurodegenerative conditions, making it a valuable target for research and potential therapeutic interventions.
With its high specificity and reproducibility, the Human NFRKB CLIA Kit offers researchers a powerful tool for studying the functions and implications of NFRKB in health and disease. Trust in this kit for accurate and precise measurements, providing valuable insights into the complex mechanisms governed by NFRKB in various biological contexts.
Product Name:
Human NFRkB (Nuclear Factor Related To Kappa B Binding Protein) CLIA Kit
SKU:
HUES01259
Target:
Human NFRkB (Nuclear Factor Related To Kappa B Binding Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NFRκB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NFRκB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NFRκB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NFRκB. You can calculate the concentration of Human NFRκB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-106
102-119
87-101
Average (%)
100
110
93
1:4
Range (%)
101-115
94-109
87-99
Average (%)
109
101
93
1:8
Range (%)
91-104
95-111
94-108
Average (%)
96
101
100
1:16
Range (%)
90-104
90-104
93-109
Average (%)
96
98
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-109
100
EDTA plasma (n=5)
95-111
101
Cell culture media (n=5)
95-109
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.13
3.62
19.58
2.12
3.87
21.4
Standard deviation
0.2
0.27
1.61
0.23
0.42
2.24
C V (%)
9.39
7.46
8.22
10.85
10.85
10.47
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human NFRκB concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NFRκB in samples. No significant cross-reactivity or interference between Human NFRκB and analogues was observed.
