Human NFE2L2 (Nuclear Factor, Erythroid Derived 2 Like 2) ELISA Kit
The Human NFE2L2 (Nuclear Factor Erythroid-Derived 2-like 2) ELISA Kit is specially designed for the precise quantification of NFE2L2 levels in human serum, plasma, and cell culture supernatants. This ELISA kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results for a variety of research applications. NFE2L2 is a key transcription factor that plays a crucial role in regulating the body's antioxidant response. It helps to protect cells from oxidative stress and inflammation by activating a network of genes involved in detoxification and defense mechanisms.
Dysregulation of NFE2L2 has been linked to various diseases such as cancer, neurodegenerative disorders, and inflammatory conditions, making it a valuable biomarker for studying these diseases and potential therapeutic interventions. The Human NFE2L2 ELISA Kit from Assay Genie provides researchers with a powerful tool to study the role of NFE2L2 in health and disease, offering accurate and reliable measurements of NFE2L2 levels in biological samples. Trust in the precision and quality of Assay Genie's ELISA kits for your research needs.
Product Name:
Human NFE2L2 (Nuclear Factor, Erythroid Derived 2 Like 2) ELISA Kit
SKU:
HUES02578
Target:
Human NFE2L2 (Nuclear Factor, Erythroid Derived 2 Like 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NFE2L2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NFE2L2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NFE2L2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NFE2L2. You can calculate the concentration of Human NFE2L2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-111
98-111
94-108
Average (%)
104
104
102
1:4
Range (%)
92-107
86-97
81-93
Average (%)
98
92
88
1:8
Range (%)
86-100
83-96
87-100
Average (%)
93
88
94
1:16
Range (%)
92-105
87-99
86-98
Average (%)
100
93
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
98
EDTA plasma (n=5)
90-103
96
Cell culture media (n=5)
87-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.53
1.49
3.38
0.56
1.38
3.25
Standard deviation
0.03
0.06
0.17
0.04
0.07
0.12
C V (%)
5.66
4.03
5.03
7.14
5.07
3.69
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human NFE2L2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NFE2L2 in samples. No significant cross-reactivity or interference between Human NFE2L2 and analogues was observed.
