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Human NF-kBp65 (Nuclear Factor Kappa B p65) CLIA Kit (HUES00797)

SKU:
HUES00797
Product Type:
ELISA Kit
ELISA Type:
CLIA Kit
Size:
96 Assays
Sensitivity:
18.75pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
NFKB3, p65, RelA
Reactivity:
Human
Sample Type:
Serum, plasma and other biological fluids
Research Area:
Immunology
€649
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human NF-kBp65 (Nuclear Factor Kappa B p65) CLIA Kit
SKU:HUES00797
Target:Human NF-kBp65 (Nuclear Factor Kappa B p65)
Size:96T
Assay type:Sandwich
Assay time:4.5h
Sensitivity:18.75 pg/mL
Detection range:31.25-2000 pg/mL
Kit component:
ComponentQuantity (24 Assays)Quantity (96 Assays)Storage
CLIA Plate8 wells x 3 strips8 wells x 12 strips-20°C, 6 months
Reference Standard1 vial2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 60 µL1 vial, 120 µL
Concentrated HRP Conjugate (100×)1 vial, 60 µL1 vial, 120 µL-20°C (shading light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL1 vial, 20 mL4°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL1 vial, 30 mL
Substrate Reagent A1 vial, 5 mL1 vial, 5 mL4°C (shading light)
Substrate Reagent B1 vial, 5 mL1 vial, 5 mL
Desiccant11
Plate Sealer5 pieces5 pieces
Product Description1 copy1 copy

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NF-κB p65. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NF-κB p65 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NF-κB p65, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NF-κB p65. You can calculate the concentration of Human NF-κB p65 in the samples by comparing the RLU value of the samples to the standard curve.

Linearity:
Serum (n=5)EDTA plasma (n=5)Cell culture media (n=5)
1:2Range (%)93-10992-10487-99
Average (%)1009892
1:4Range (%)101-11596-111100-113
Average (%)107103107
1:8Range (%)101-11896-10898-116
Average (%)108102106
1:16Range (%)88-9885-9895-109
Average (%)9390100
Recovery:
Sample TypeRange (%)Average Recovery (%)
Serum (n=5)97-110104
EDTA plasma (n=5)99-113105
Cell culture media (n=5)89-10396
Precision:
Intra-assay PrecisionInter-assay Precision
Sample123123
n20.020.020.020.020.020.0
Mean (ng/mL)107.99203.7965.39100.87215.26983.84
Standard deviation11.5723.3481.0911.8420.5696.22
C V (%)10.7111.468.411.749.559.78
Sample type &Sample volume:Serum, plasma and other biological fluids; 100μL
Reproducibility:Both intra-CV and inter-CV are < 15%.
Application:This CLIA kit applies to the in vitro quantitative determination of Human NF-κB p65 concentrations in Serum, plasma and other biological fluids.
Specificity:This kit recognizes Human NF-κB p65 in samples. No significant cross-reactivity or interference between Human NF-κB p65 and analogues was observed.

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