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Human NF-kB Pathway Phosphorylation Array (11 targets) (SARB0059)

SKU:
SARB0059
Product Type:
Phosphorylation Array
Analytes:
ATM (P-Ser1981)
eIF-2a (P-Ser52)
HDAC2 (P-Ser394)
HDAC4 (P-Ser632)
IKB-alpha (P-Ser32)
MSK1 (P-Ser376)
NF-kB (P-Ser536)
STAT1 (P-Ser727)
TAK1 (P-Ser412)
TBK1 (P-Ser172)
ZAP70 (P-Tyr292)
Reactivity:
Human
Applications:
Multiplex Array
€499 - €959
Frequently bought together:

Description

system_update_altDatasheet

Human NF-kB Pathway Phosphorylation Array (11 targets) (SARB0059)

The Human NF-kB Pathway Phosphorylation Array (SARB0059) offered by AssayGenie is a powerful tool for studying the activation of the NF-kB signaling pathway. This array contains antibodies specific to 11 target proteins involved in the NF-kB pathway, allowing for the simultaneous detection and analysis of phosphorylation events.The NF-kB pathway is crucial for regulating immune responses, inflammation, and cell survival, making it a key focus in research related to cancer, autoimmune diseases, and inflammatory conditions. By using the Human NF-kB Pathway Phosphorylation Array, researchers can gain insights into the activation status of key proteins in this pathway, facilitating the identification of potential drug targets and biomarkers for disease diagnosis and prognosis.

This array is compatible with various sample types, including cell lysates and tissue homogenates, and its high sensitivity and specificity ensure reliable and reproducible results. Whether studying basic mechanisms of cell signaling or exploring potential therapeutic interventions, the Human NF-kB Pathway Phosphorylation Array is a valuable resource for researchers in immunology, oncology, and beyond.

Product Sku:SARB0059
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:11
Gene Symbols:ATM, EIF2S1, HDAC2, HDAC4, MAP3K7, NFKBIA, RELA, RPS6KA5, STAT1, TBK1|ZAP70
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:MAPK Signaling / mTOR Signaling / NFkB Signaling / p53 Signaling / PKC Signaling
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
ATM (P-Ser1981)
  • Gene Symbol: ATM
  • Gene ID: 472;
  • Accession #: Q13315
eIF-2a (P-Ser52)
  • Gene Symbol: EIF2S1 EIF2A
  • Gene ID: 1965;
  • Accession #: P05198
HDAC2 (P-Ser394)
  • Gene Symbol: HDAC2
  • Gene ID: 3066
  • Accession #: Q92769
HDAC4 (P-Ser632)
  • Gene Symbol: HDAC4 KIAA0288
  • Gene ID: 9759;
  • Accession #: P56524
IKB-alpha (P-Ser32)
  • Gene Symbol: NFKBIA IKBA MAD3 NFKBI
  • Gene ID: 4792;
  • Accession #: P25963
MSK1 (P-Ser376)
  • Gene Symbol: RPS6KA5 MSK1
  • Gene ID: 9252;
  • Accession #: O75582
NF-kB (P-Ser536)
  • Gene Symbol: RELA NFKB3
  • Gene ID: 5970;
  • Accession #: Q04206
STAT1 (P-Ser727)
  • Gene Symbol: STAT1
  • Gene ID: 6772;
  • Accession #: P42224
TAK1 (P-Ser412)
  • Gene Symbol: MAP3K7 TAK1
  • Gene ID: 6885;
  • Accession #: O43318
TBK1 (P-Ser172)
  • Gene Symbol: TBK1 NAK
  • Gene ID: 29110;
  • Accession #: Q9UHD2
ZAP70 (P-Tyr292)
  • Gene Symbol: ZAP70 SRK
  • Gene ID: 7535;
  • Accession #: P43403
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Human NF-kB Pathway Phosphorylation Array C1 Membranes
  • Blocking Buffer
  • Detection Antibody Cocktail
  • 1,000X HRP-Anti-Rabbit-IgG Concentrate
  • 20X Wash Buffer I Concentrate
  • 20X Wash Buffer II Concentrate
  • 2X Cell Lysis Buffer Concentrate
  • Detection Buffer C
  • Detection Buffer D
  • 8-Well Incubation Tray w/ Lid
  • Protease Inhibitor Cocktail
  • 100x Phosphatase Inhibitor Cocktail I
  • Phosphatase Inhibitor Cocktail II
  • Plastic Sheets
  • Array Map Template

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or plastic Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system: CCD camera, X-ray Film and a suitable film processor, gel documentation system, or another chemiluminescent detection system capable of imaging a western blot.
1.Block membranes
2.Incubate with Sample
3.Incubate with Detection Antibody Cocktail
4.Incubate with HRP-Conjugated anti-IgG
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis

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