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Human Neutrophil Gelatinase Associated Lipocalin (NGAL) ELISA Kit

SKU:
HUDL02031
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P80188
Sensitivity:
0.06ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
LCN2,p25,Lipocalin 2,Oncogene 24p3,25 kDa alpha-2-microglobulin-related subunit of MMP-9,Siderocalin LCN2
Reactivity:
Human
Sample Type:
Serum, Plasma, Urine, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€599
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Description

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Human Neutrophil Gelatinase Associated Lipocalin (NGAL) ELISA Kit

The Human Neutrophil Gelatinase-Associated Lipocalin (NGAL) ELISA Kit is specifically designed for the accurate measurement of NGAL levels in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it ideal for various research applications.NGAL is a protein that plays a critical role in the early detection of kidney injury and is also associated with various inflammatory and infectious conditions.

Monitoring NGAL levels can provide valuable insights into kidney health and overall disease progression, making it a valuable biomarker for diagnostic and therapeutic purposes.With easy-to-follow protocols and quick assay times, the Human NGAL ELISA Kit from Assay Genie is a valuable tool for researchers and clinicians studying kidney function and related diseases. Get accurate results and valuable data with this innovative ELISA kit.

Product Name:Human Neutrophil Gelatinase Associated Lipocalin (NGAL) ELISA Kit
Product Code:HUDL02031
Size:96 Assays
Target:NGAL
Synonyms:LCN2, p25, Lipocalin 2, Oncogene 24p3, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, Siderocalin LCN2
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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