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Human Neurotensin Receptor 1,High Affinity (NTSR1) ELISA Kit

SKU:
HUDL02111
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P30989
Sensitivity:
0.126ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
NTR,NTRH,NTRR,High-affinity levocabastine-insensitive neurotensin receptor
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Human Neurotensin Receptor 1,High Affinity (NTSR1) ELISA Kit

The Human Neurotensin Receptor 1 (NTSR1) High Affinity ELISA Kit is a cutting-edge tool designed for the precise detection of NTSR1 levels in human samples. This kit offers exceptional sensitivity and specificity, guaranteeing trustworthy and consistent results for various research purposes.NTSR1 is a critical receptor involved in neurotensin signaling, a peptide that regulates numerous physiological processes in the central nervous system. Dysregulation of NTSR1 has been linked to various neurological disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.

With the Human NTSR1 High Affinity ELISA Kit, researchers can accurately measure NTSR1 levels in serum, plasma, and cell culture supernatants, enabling detailed investigations into the role of NTSR1 in health and disease. Trust this innovative kit for robust and accurate NTSR1 analysis in your research endeavors.

Product Name:Human Neurotensin Receptor 1,High Affinity (NTSR1) ELISA Kit
Product Code:HUDL02111
Size:96 Assays
Target:NTSR1
Synonyms:NTR, NTRH, NTRR, High-affinity levocabastine-insensitive neurotensin receptor
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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