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Human Neuroepithelial Cell Transforming Gene 1 (NET1) ELISA Kit

SKU:
HUDL02016
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q7Z628
Sensitivity:
0.125ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
ARHGEF8,NET1A,Proto-oncogene p65 Net1,Rho guanine nucleotide exchange factor 8
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
$779
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Description

system_update_altDatasheet

Human Neuroepithelial Cell Transforming Gene 1 (NET1) ELISA Kit

The Human NET1 (Neuroepithelial Cell Transforming Gene 1) ELISA Kit is a powerful tool for the precise measurement of NET1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit is renowned for its exceptional sensitivity and specificity, ensuring dependable and consistent results for a variety of research applications.NET1 is a key player in regulating cell transformation and has been implicated in a range of diseases, including cancer, cardiovascular issues, and neurodegenerative disorders.

By accurately quantifying NET1 levels, researchers can gain valuable insights into the molecular mechanisms underlying these conditions, ultimately leading to the development of novel therapeutic strategies. Elevate your research with the Human NET1 ELISA Kit from Assay Genie.

Product Name:Human Neuroepithelial Cell Transforming Gene 1 (NET1) ELISA Kit
Product Code:HUDL02016
Size:96 Assays
Target:NET1
Synonyms:ARHGEF8, NET1A, Proto-oncogene p65 Net1, Rho guanine nucleotide exchange factor 8
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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