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Human Neuregulin 4 (NRG4) ELISA Kit

SKU:
HUDL02091
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q0P6N6
Sensitivity:
0.31ng/mL
Range:
0.781-50ng/mL
ELISA Type:
Sandwich
Synonyms:
HRG4,Pro-NRG4,Pro-neuregulin-4, membrane-bound isoform
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Neuregulin 4 (NRG4) ELISA Kit

The Human Neuregulin-4 (NRG4) ELISA Kit is a reliable and accurate tool for measuring levels of NRG4 in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides precise and reproducible results, making it ideal for various research applications.NRG4 is a vital protein involved in regulating metabolism, insulin sensitivity, and energy balance. It serves as a potential biomarker for metabolic disorders such as obesity, diabetes, and cardiovascular diseases, making it a valuable target for studying these conditions and developing new therapeutic strategies.

Overall, the Human NRG4 ELISA Kit offers researchers a powerful tool for investigating the role of NRG4 in metabolic regulation and its potential implications for disease diagnosis and treatment.

Product Name:Human Neuregulin 4 (NRG4) ELISA Kit
Product Code:HUDL02091
Size:96 Assays
Target:NRG4
Synonyms:HRG4, Pro-NRG4, Pro-neuregulin-4, membrane-bound isoform
Detection Method:Sandwich
Range:0.781-50ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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