SYNE1,C6orf98,8B,MYNE1,CPG2,SCAR8,ARCA1,Enaptin,Myocyte nuclear envelope protein 1,Nuclear envelope spectrin repeat protein 1,Synaptic nuclear envelope protein 1
The Human Nesprin-1 (Nesp1) ELISA Kit is a powerful tool for the precise measurement of Nesprin-1 levels in human samples including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable asset for various research applications.Nesprin-1 is a critical component involved in cellular structure and function, particularly in the organization of the nuclear envelope and cytoskeleton.
Dysregulation of Nesprin-1 has been linked to various diseases such as muscular dystrophy, Emery-Dreifuss muscular dystrophy, and cancer, highlighting its importance as a biomarker for disease research and potential therapeutic targets.Don't miss out on the opportunity to study Nesprin-1 and its implications in human health and disease with the reliable and efficient Human Nesprin-1 ELISA Kit. Order yours today from AssayGenie and take your research to the next level.
Product Name:
Human Nesprin 1 (Nesp1) ELISA Kit
Product Code:
HUDL02014
Size:
96 Assays
Target:
Nesp1
Synonyms:
SYNE1, C6orf98, 8B, MYNE1, CPG2, SCAR8, ARCA1, Enaptin, Myocyte nuclear envelope protein 1, Nuclear envelope spectrin repeat protein 1, Synaptic nuclear envelope protein 1
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
89-101
95
EDTA plasma (n=5)
91-101
96
Heparin plasma (n=5)
91-101
96
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
