Human NENF Recombinant Protein (RPPB0782)
- SKU:
- RPPB0782
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9UMX5
- Research Area:
- Neurotrophins
Description
| Product Name: | Human NENF Recombinant Protein |
| Product Code: | RPPB0782 |
| Size: | 10µg |
| Species: | Human |
| Target: | NENF |
| Synonyms: | Neudesin, Cell immortalization-related protein 2, Neuron-derived neurotrophic factor, Secreted protein of unknown function, SPUF protein, NENF, CIR2, SPUF, SCIRP10. |
| Source: | Escherichia Coli |
| Physical Appearance: | Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | NENF was filtered (0.4�m) and lyophilized from 0.5mg/ml in 0.05M phosphate buffer and 0.075M NaCl, pH 7.4. |
| Solubility: | It is recommended to add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. NENF is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. |
| Stability: | Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MKHHHHHHASGQTPRPAERG PPVRLFTEEE LARYGGEEED QPIYLAVKGV VFDVTSGKEF YGRGAPYNAL TGKDSTRGVA KMSLDPADLT HDTTGLTAKE LEALDEVFTK VYKAKYPIVG YTARRILNED GSPNLDFKPE DQPHFDIKDE F |
Neudesin Neurotrophic Factor (NENF) is a member of the cytochrome b5 family, MAPR subfamily. NENF contains 1 cytochrome b5 heme-binding domain. NENF exhibits neurotrophic activity and activates phosphorylation of MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT in primary cultured neurons. NENF doesn�t have mitogenic activity in primary cultured astrocytes. NENF may play a part in neuronal differentiation and may have a transient influence on neural cell proliferation in neural precursor cells. NENF neurotrophic activity is increased by binding to heme. NENF is up-regulated in immortal cells and induced in estrogen receptor positive breast cancer expressing progesterone receptor.
NENF Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (aa 32-172) containing 151 amino acids including a 10 a.a N-terminal His tag�and having a molecular mass of 16.9kDa.
| UniProt Protein Function: | NENF: Displays neurotrophic activity and activates phosphorylation of MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT in primary cultured neurons. Does not have mitogenic activity in primary cultured astrocytes. May play a role on neuronal differentiation and may have a transient effect on neural cell proliferation in neural precursor cells. Neurotrophic activity is enhanced by binding to heme. Belongs to the cytochrome b5 family. MAPR subfamily.Protein type: Secreted; Secreted, signal peptideChromosomal Location of Human Ortholog: 1q32.3Cellular Component: endomembrane system; extracellular space; membraneMolecular Function: growth factor activity; metal ion bindingBiological Process: negative regulation of appetite; positive regulation of MAPK cascade |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a neurotrophic factor that may play a role in neuron differentiation and development. A pseudogene of this gene is found on chromosome 12. Alternate splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2009] |
| UniProt Code: | Q9UMX5 |
| NCBI GenInfo Identifier: | 7019545 |
| NCBI Gene ID: | 29937 |
| NCBI Accession: | NP_037481.1 |
| UniProt Secondary Accession: | Q9UMX5,Q53FZ6, Q5TM90, A1KYQ8 |
| UniProt Related Accession: | Q9UMX5 |
| Molecular Weight: | 18,856 Da |
| NCBI Full Name: | neudesin |
| NCBI Synonym Full Names: | neudesin neurotrophic factor |
| NCBI Official Symbol: | NENF�� |
| NCBI Official Synonym Symbols: | CIR2; SPUF; SCIRP10�� |
| NCBI Protein Information: | neudesin |
| UniProt Protein Name: | Neudesin |
| UniProt Synonym Protein Names: | Cell immortalization-related protein 2; Neuron-derived neurotrophic factor; Protein GIG47 |
| Protein Family: | Neudesin |
| UniProt Gene Name: | NENF�� |
| UniProt Entry Name: |
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