The Human NE (Elastase-2 Neutrophil) ELISA Kit is specifically developed for the precise quantification of elastase-2 neutrophil levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results for a variety of research purposes.Elastase-2 neutrophil, also known as NE, is a key enzyme involved in immune response regulation and inflammatory processes. Abnormal levels of NE have been linked to various diseases such as chronic obstructive pulmonary disease (COPD), cystic fibrosis, and acute respiratory distress syndrome (ARDS), highlighting its importance as a biomarker in clinical research.
With the Human NE ELISA Kit, researchers can reliably measure NE levels in human samples, facilitating the study of neutrophil function and its implications in disease pathogenesis. This kit is a valuable tool for investigating potential therapeutic targets and developing innovative treatment strategies for NE-related disorders.
Product Name:
Human NE/ELA2 (Elastase 2, Neutrophil) ELISA Kit
SKU:
HUES02889
Target:
Human NE/ELA2 (Elastase 2, Neutrophil)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NE/ELA2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NE/ELA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NE/ELA2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NE/ELA2. You can calculate the concentration of Human NE/ELA2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-98
91-107
95-109
Average (%)
92
99
101
1:4
Range (%)
91-106
83-95
82-93
Average (%)
99
88
88
1:8
Range (%)
92-104
82-93
87-102
Average (%)
98
87
93
1:16
Range (%)
93-106
86-100
89-99
Average (%)
98
92
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
94
EDTA plasma (n=5)
88-100
94
Cell culture media (n=5)
93-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.58
5.84
23.39
2.53
6.4
25.58
Standard deviation
0.13
0.31
0.91
0.17
0.28
1.21
C V (%)
5.04
5.31
3.89
6.72
4.38
4.73
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human NE/ELA2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NE/ELA2 in samples. No significant cross-reactivity or interference between Human NE/ELA2 and analogues was observed.
