Human NCK2 Recombinant Protein (RPPB4066)
- SKU:
- RPPB4066
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- O43639
Description
| Product Name: | Human NCK2 Recombinant Protein |
| Product Code: | RPPB4066 |
| Size: | 10µg |
| Species: | Human |
| Target: | NCK2 |
| Synonyms: | NCK Adaptor Protein 2, Growth Factor Receptor-Bound Protein 4, SH2/SH3 Adaptor Protein NCK-Beta, Noncatalytic Region Of Tyrosine Kinase Beta, �Cytoplasmic Protein NCK2, GRB4, NCKbeta, Nck-2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The NCK2 solution contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 1mM DTT and 30% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.�Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMTEEVIV IAKWDYTAQQ DQELDIKKNE RLWLLDDSKT WWRVRNAANR TGYVPSNYVE RKNSLKKGSL VKNLKDTLGL GKTRRKTSAR DASPTPSTDA EYPANGSGAD RIYDLNIPAF VKFAYVAERE DELSLVKGSR VTVMEKCSDG WWRGSYNGQI GWFPSNYVLE EVDEAAAESP SFLSLRKGAS LSNGQGSRVL HVVQTLYPFS SVTEEELNFE KGETMEVIEK PENDPEWWKC KNARGQVGLV PKNYVVVLSD GPALHPAHAP QISYTGPSSS GRFAGREWYY GNVTRHQAEC ALNERGVEGD FLIRDSESSP SDFSVSLKAS GKNKHFKVQL VDNVYCIGQR RFHTMDELVE HYKKAPIFTS EHGEKLYLVR ALQ |
NCK2 belongs to the NCK family of adaptor proteins. NCK2 holds one SH2 domain and three SH3 domains. NCK2 bind and recruit several proteins that take part in the regulation of receptor protein tyrosine kinases even though it has no known catalytic function. That indicates that NCK2 takes part in cytoskeletal reorganization. Alternate transcription splice variants, encoding diverse isoforms, were characterized.
NCK2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 403 amino acids (1-380) and having a molecular mass of 45.3kDa.NCK2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | NCK2: Adapter protein which associates with tyrosine- phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1- dependent transcriptional activation in response to activated Ras signaling. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 2q12 Cellular Component: endoplasmic reticulum; cytoplasm; vesicle membrane; cytosol Molecular Function:protein binding; receptor signaling complex scaffold activity; SH3/SH2 adaptor activity; cytoskeletal adaptor activity Biological Process: epidermal growth factor receptor signaling pathway; lamellipodium biogenesis; axon guidance; cell migration; T cell activation; positive regulation of signal transduction; regulation of epidermal growth factor receptor activity; negative regulation of peptidyl-serine phosphorylation; signal complex assembly; actin filament organization; signal transduction; regulation of translation; negative regulation of cell proliferation; positive regulation of actin filament polymerization; ephrin receptor signaling pathway; positive regulation of T cell proliferation; positive regulation of transcription from RNA polymerase II promoter; vascular endothelial growth factor receptor signaling pathway |
| NCBI Summary: | This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O43639 |
| NCBI GenInfo Identifier: | 20532395 |
| NCBI Gene ID: | 8440 |
| NCBI Accession: | O43639.2 |
| UniProt Secondary Accession: | O43639,Q9BWN9, Q9UIC3, D3DVK1, |
| UniProt Related Accession: | O43639 |
| Molecular Weight: | 380 |
| NCBI Full Name: | Cytoplasmic protein NCK2 |
| NCBI Synonym Full Names: | NCK adaptor protein 2 |
| NCBI Official Symbol: | NCK2�� |
| NCBI Official Synonym Symbols: | GRB4; NCKbeta�� |
| NCBI Protein Information: | cytoplasmic protein NCK2; SH2/SH3 adaptor protein NCK-beta; growth factor receptor-bound protein 4; noncatalytic region of tyrosine kinase, beta |
| UniProt Protein Name: | Cytoplasmic protein NCK2 |
| UniProt Synonym Protein Names: | Growth factor receptor-bound protein 4; NCK adaptor protein 2; Nck-2; SH2/SH3 adaptor protein NCK-beta |
| Protein Family: | Cytoplasmic protein |
| UniProt Gene Name: | NCK2�� |
| UniProt Entry Name: | NCK2_HUMAN |
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