Human NCALD Recombinant Protein (RPPB4060)
- SKU:
- RPPB4060
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P61601
Description
| Product Name: | Human NCALD Recombinant Protein |
| Product Code: | RPPB4060 |
| Size: | 20µg |
| Species: | Human |
| Target: | NCALD |
| Synonyms: | Rocalcin Delta, MGC33870, MGC74858, Neurocalcin-Delta. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The NCALD protein solution (0.5mg/1ml) is formulated in 20 mM Tris-HCl buffer (pH8.0), 100mM NaCl, 1mM DTT and 40% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGKQNSKLRP EVMQDLLEST DFTEHEIQEW YKGFLRDCPS GHLSMEEFKK IYGNFFPYGD ASKFAEHVFR TFDANGDGTI DFREFIIALS VTSRGKLEQK LKWAFSMYDL DGNGYISKAE MLEIVQAIYK MVSSVMKMPE DESTPEKRTE KIFRQMDTNR DGKLSLEEFI RGAKSDPSIV RLLQCDPSSA GQF |
NCALD belongs to the neuronal calcium sensor (NCS) family. NCALD is cytosolic at inactive calcium levels; but, high intracellular calcium levels induce a conformational transformation which exposes the myristoyl group, causing protein association with membranes and partial co-localization with the perinuclear trans-golgi network. NCALD protein is a regulator of signal transduction by G protein-coupled receptor.
NCALD produced in E.Coli is a single, non-glycosylated polypeptide chain containing 213 amino acids (1-193.a.a) and having a molecular mass of 24.4kDa. NCALD is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | NCALD: May be involved in the calcium-dependent regulation of rhodopsin phosphorylation. Binds three calcium ions. Belongs to the recoverin family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 8q22.2 Cellular Component: clathrin coat of trans-Golgi network vesicle; intracellular; cytosol Molecular Function:tubulin binding; protein binding; clathrin binding; calcium ion binding; actin binding; alpha-tubulin binding Biological Process: vesicle-mediated transport; synaptic transmission; regulation of systemic arterial blood pressure; calcium-mediated signaling |
| NCBI Summary: | This gene encodes a member of the neuronal calcium sensor (NCS) family of calcium-binding proteins. The protein contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein is cytosolic at resting calcium levels; however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. The protein is thought to be a regulator of G protein-coupled receptor signal transduction. Several alternatively spliced variants of this gene have been determined, all of which encode the same protein; additional variants may exist but their biological validity has not been determined. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P61601 |
| NCBI GenInfo Identifier: | 47606440 |
| NCBI Gene ID: | 83988 |
| NCBI Accession: | P61601.2 |
| UniProt Related Accession: | P61601 |
| Molecular Weight: | |
| NCBI Full Name: | Neurocalcin-delta |
| NCBI Synonym Full Names: | neurocalcin delta |
| NCBI Official Symbol: | NCALD�� |
| NCBI Protein Information: | neurocalcin-delta |
| UniProt Protein Name: | Neurocalcin-delta |
| Protein Family: | Neurocalcin |
| UniProt Gene Name: | NCALD�� |
| UniProt Entry Name: | NCALD_HUMAN |
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