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Human Natriuretic Peptide Receptor 3 (NPR3) ELISA Kit

SKU:
HUDL02073
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P17342
Sensitivity:
0.059ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
GUCY2B,NPRC,ANPRC,Guanylate Cyclase C,Atrionatriuretic Peptide Receptor C,Atrial natriuretic peptide clearance receptor
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

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Human Natriuretic Peptide Receptor 3 (NPR3) ELISA Kit

The Human Natriuretic Peptide Receptor 3 (NPR3) ELISA Kit is a powerful tool designed for precise detection of NPR3 levels in human samples including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers dependable and consistent results, making it perfect for a wide array of research studies.NPR3 is a vital receptor involved in the regulation of natriuretic peptides, playing a crucial role in cardiovascular physiology and pathology.

Its dysregulation has been linked to various cardiovascular diseases, making it a valuable biomarker for studying and understanding these conditions. This ELISA kit is essential for researchers looking to investigate NPR3 function and potentially develop therapeutic interventions in cardiovascular health. Get your hands on the Human NPR3 ELISA Kit today and take your research to the next level.

Product Name:Human Natriuretic Peptide Receptor 3 (NPR3) ELISA Kit
Product Code:HUDL02073
Size:96 Assays
Target:NPR3
Synonyms:GUCY2B, NPRC, ANPRC, Guanylate Cyclase C, Atrionatriuretic Peptide Receptor C, Atrial natriuretic peptide clearance receptor
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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