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Human NANS Recombinant Protein (RPPB1997)

SKU:
RPPB1997
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9NR45
Research Area:
Enzymes
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Description

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Product Name:Human NANS Recombinant Protein
Product Code:RPPB1997
Size:20µg
Species:Human
Target:NANS
Synonyms:Sialic acid synthase, N-acetylneuraminate synthase, N-acetylneuraminate-9-phosphate synthase, N-acetylneuraminic acid phosphate synthase, N-acetylneuraminic acid synthase, NANS, SAS.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The NANS solution (0.5 mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 0.1M NaCl, 1mM DTT and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MPLELELCPG RWVGGQHPCF IIAEIGQNHQ GDLDVAKRMI RMAKECGADC AKFQKSELEF KFNRKALERP YTSKHSWGKT YGEHKRHLEF SHDQYRELQR YAEEVGIFFT ASGMDEMAVE FLHELNVPFF KVGSGDTNNF PYLEKTAKKG RPMVISSGMQ SMDTMKQVYQ IVKPLNPNFC FLQCTSAYPL QPEDVNLRVI SEYQKLFPDI PIGYSGHETG IAISVAAVAL GAKVLERHIT LDKTWKGSDH SASLEPGELA ELVRSVRLVE RALGSPTKQL LPCEMACNEK LGKSVVAKVK IPEGTILTMD MLTVKVGEPK GYPPEDIFNL VGKKVLVTVE EDDTIMEELV DNHGKKIKS

NANS is a 359 amino acid protein that contains one AFP (antifreeze proteins)-like domain and functions in the biosynthesis of sialic acids. The ubiquitously expressed NANS enzymatically catalyzes the H2O-dependent formation of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), both of which are sialic acids. NANS uses N-acetylmannosamine 6-phosphate as a substrate for Neu5Ac synthesis and mannose 6-phosphate as a substrate for KDN synthesis.

NANS Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 379 amino acids (1-359 a.a.) and having a molecular mass of 42.4kDa. The NANS is purified by proprietary chromatographic techniques.

UniProt Protein Function:NANS: Produces N-acetylneuraminic acid (Neu5Ac) and 2-keto-3- deoxy-D-glycero-D-galacto-nononic acid (KDN). Can also use N- acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of Neu5Ac and KDN, respectively.
UniProt Protein Details:

Protein type:Carbohydrate Metabolism - amino sugar and nucleotide sugar; EC 2.5.1.57; Transferase; EC 2.5.1.56

Chromosomal Location of Human Ortholog: 9p24.1-p23

Cellular Component: cytoplasm; cytosol

Molecular Function:N-acylneuraminate-9-phosphate synthase activity; N-acetylneuraminate synthase activity; N-acylneuraminate cytidylyltransferase activity

Biological Process: cellular protein metabolic process; dolichol-linked oligosaccharide biosynthetic process; lipopolysaccharide biosynthetic process; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification

NCBI Summary:This gene encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively; however, it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells, expression of this gene results in Neu5Ac and KDN production. This gene is related to the E. coli sialic acid synthase gene neuB, and it can partially restore sialic acid synthase activity in an E. coli neuB-negative mutant. [provided by RefSeq, Jul 2008]
UniProt Code:Q9NR45
NCBI GenInfo Identifier:20978759
NCBI Gene ID:54187
NCBI Accession:Q9NR45.2
UniProt Secondary Accession:Q9NR45,Q8WUV9, Q9BWS6, Q9NVD4, B2RE98,
UniProt Related Accession:Q9NR45
Molecular Weight:359
NCBI Full Name:Sialic acid synthase
NCBI Synonym Full Names:N-acetylneuraminic acid synthase
NCBI Official Symbol:NANS��
NCBI Official Synonym Symbols:SAS; HEL-S-100��
NCBI Protein Information:sialic acid synthase; N-acetylneuraminate synthase; sialic acid phosphate synthase; epididymis secretory protein Li 100; N-acetylneuraminate-9-phosphate synthase; N-acetylneuraminic acid phosphate synthase
UniProt Protein Name:Sialic acid synthase
UniProt Synonym Protein Names:N-acetylneuraminate synthase (EC:2.5.1.56); N-acetylneuraminate-9-phosphate synthase (EC:2.5.1.57); N-acetylneuraminic acid phosphate synthase; N-acetylneuraminic acid synthase
Protein Family:Sialic acid synthase
UniProt Gene Name:NANS��
UniProt Entry Name:SIAS_HUMAN

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