Human NAGase (N-Acetyl Beta-D-Glucosaminidase) ELISA Kit
The Human NAGASE (N-acetyl-beta-D-glucosaminidase) ELISA Kit is a reliable tool for the precise measurement of NAGASE levels in human samples including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers consistent and accurate results, making it suitable for a diverse range of research purposes.NAGASE is an important enzyme involved in the breakdown of complex sugars, playing a key role in various biological processes. Its dysregulation has been linked to several diseases such as lysosomal storage disorders and renal diseases.
By measuring NAGASE levels, researchers can gain valuable insights into these conditions and explore potential therapeutic interventions.This ELISA kit provides a convenient and efficient solution for studying NAGASE and its implications in human health and disease. Trust in its performance to advance your research and discover new possibilities in the field of biochemistry.
Product Name:
Human NAGase (N-Acetyl Beta-D-Glucosaminidase) ELISA Kit
SKU:
HUES02024
Target:
Human NAGase (N-Acetyl Beta-D-Glucosaminidase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NAGase. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NAGase and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NAGase, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NAGase. You can calculate the concentration of Human NAGase in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-101
93-106
89-102
Average (%)
92
101
96
1:4
Range (%)
91-107
84-98
86-101
Average (%)
98
90
92
1:8
Range (%)
89-103
80-94
86-99
Average (%)
96
86
91
1:16
Range (%)
85-100
82-92
86-99
Average (%)
92
87
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
99
EDTA plasma (n=5)
92-106
99
Cell culture media (n=5)
95-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.4
13.62
47.6
5.69
13.4
46.83
Standard deviation
0.37
0.61
1.49
0.33
0.68
1.77
C V (%)
6.85
4.48
3.13
5.8
5.07
3.78
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human NAGase concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NAGase in samples. No significant cross-reactivity or interference between Human NAGase and analogues was observed.
