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Human NADH Dehydrogenase, Quinone 1 (NQO1) ELISA Kit

SKU:
HUDL02084
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P15559
Sensitivity:
0.124ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
DHQU,DIA4,DTD,NMOR1,NMORI,QR1,Azoreductase,DT-diaphorase,Menadione reductase,Phylloquinone reductase,Quinone reductase 1,NAD(P)H:quinone oxidoreductase 1
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Human NADH Dehydrogenase, Quinone 1 (NQO1) ELISA Kit

The Human NQO1 (NADH dehydrogenase quinone 1) ELISA Kit is a powerful tool for the precise measurement of NQO1 levels in human samples. This kit is highly sensitive and specific, ensuring accurate and consistent results that are essential for various research applications.NQO1 is an important enzyme involved in detoxification pathways and oxidative stress responses. It plays a critical role in protecting cells from damage and has been associated with various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases.

The ability to measure NQO1 levels accurately can provide valuable insights into the progression and treatment of these conditions.With its user-friendly protocols and reliable performance, the Human NQO1 ELISA Kit is a valuable resource for researchers looking to study NQO1 levels in human samples and explore its potential as a biomarker for disease diagnosis and treatment monitoring.

Product Name:Human NADH Dehydrogenase, Quinone 1 (NQO1) ELISA Kit
Product Code:HUDL02084
Size:96 Assays
Target:NQO1
Synonyms:DHQU, DIA4, DTD, NMOR1, NMORI, QR1, Azoreductase, DT-diaphorase, Menadione reductase, Phylloquinone reductase, Quinone reductase 1, NAD(P)H:quinone oxidoreductase 1
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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