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Human NADH Dehydrogenase 5 (ND5) ELISA Kit

SKU:
HUDL02000
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P03915
Sensitivity:
0.072ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
MT-ND5,MTND5,NAD5,NADH-Ubiquinone Oxidoreductase Chain 5,Mitochondrially Encoded NADH Dehydrogenase 5
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€749
Frequently bought together:

Description

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Human NADH Dehydrogenase 5 (ND5) ELISA Kit

The Human NADH Dehydrogenase 5 (ND5) ELISA Kit is a powerful tool for the precise measurement of ND5 levels in human biological samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it suitable for a wide range of research applications.ND5 is a key enzyme in the mitochondrial respiratory chain, playing a vital role in energy production through oxidative phosphorylation. Dysregulation of ND5 has been linked to various diseases, including mitochondrial disorders, neurodegenerative diseases, and cancer.

Therefore, measuring ND5 levels using this ELISA kit can offer valuable insights into these conditions and aid in the development of potential therapeutic strategies.Overall, the Human NADH Dehydrogenase 5 (ND5) ELISA Kit is an essential tool for researchers studying mitochondrial function, energy metabolism, and associated diseases, providing accurate and reliable results for their research needs.

Product Name:Human NADH Dehydrogenase 5 (ND5) ELISA Kit
Product Code:HUDL02000
Size:96 Assays
Target:ND5
Synonyms:MT-ND5, MTND5, NAD5, NADH-Ubiquinone Oxidoreductase Chain 5, Mitochondrially Encoded NADH Dehydrogenase 5
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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