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Human NACA Recombinant Protein (RPPB4050)

SKU:
RPPB4050
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q13765
€309
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Description

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Product Name:Human NACA Recombinant Protein
Product Code:RPPB4050
Size:20µg
Species:Human
Target:NACA
Synonyms:Nascent polypeptide-associated complex alpha subunit, NACA1, NAC-alpha, alpha-NAC, Allergen Hom s 2.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The NACA solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 1mM DTT and 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MPGEATETVP ATEQELPQPQ AETGSGTESD SDESVPELEE QDSTQATTQQ AQLAAAAEID EEPVSKAKQS RSEKKARKAM SKLGLRQVTG VTRVTIRKSK NILFVITKPD VYKSPASDTY IVFGEAKIED LSQQAQLAAA EKFKVQGEAV SNIQENTQTP TVQEESEEEE VDETGVEVKD IELVMSQANV SRAKAVRALK NNSNDIVNAI MELTM

NACA belongs to the nascent polypeptide associated complex (NAC) alpha subunit family which takes part in inhibiting unsuitable targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). NACA proteins are usually restricted to the nucleus and cytoplasm and hold NAC-A/B (NAC-alpha/beta) and UBA (ubiquitin-associated) domains. The UBA domain is related to proteins which takes part in the ubiquitin-proteasome pathway for protein degradation.

NACA Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 235 amino acids (1-215) and having a molecular mass of 25.5 kDa.NACA is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:NACA: Prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites). May act as a specific coactivator for JUN, binding to DNA and stabilizing the interaction of JUN homodimers with target gene promoters. Belongs to the NAC-alpha family. Interacts with TBP and JUN. Part of the nascent polypeptide-associated complex (NAC), consisting of NACA and BTF3. NAC associates with ribosomes through the BTF3 subunit. Both subunits can contact nascent polypeptide chains. Interacts with ASFV protein H339R.
UniProt Protein Details:

Protein type:Transcription, coactivator/corepressor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12q23-q24.1

Cellular Component: cytoplasm; nascent polypeptide-associated complex; nucleus

Molecular Function:DNA binding; protein binding; transcription coactivator activity

Biological Process: positive regulation of skeletal muscle growth; protein transport; regulation of skeletal muscle fiber development; regulation of transcription, DNA-dependent; skeletal muscle regeneration; transcription, DNA-dependent; translation; viral reproduction

NCBI Summary:This gene encodes a protein that associates with basic transcription factor 3 (BTF3) to form the nascent polypeptide-associated complex (NAC). This complex binds to nascent proteins that lack a signal peptide motif as they emerge from the ribosome, blocking interaction with the signal recognition particle (SRP) and preventing mistranslocation to the endoplasmic reticulum. This protein is an IgE autoantigen in atopic dermatitis patients. Alternative splicing results in multiple transcript variants, but the full length nature of some of these variants, including those encoding very large proteins, has not been determined. There are multiple pseudogenes of this gene on different chromosomes. [provided by RefSeq, Feb 2016]
UniProt Code:Q13765
NCBI GenInfo Identifier:767974358
NCBI Gene ID:4666
NCBI Accession:XP_011536691.1
UniProt Related Accession:Q13765,E9PAV3
Molecular Weight:Calculated MW: 23kDa/94kDa/205kDaObserved MW: 37kDa
NCBI Full Name:nascent polypeptide-associated complex subunit alpha isoform X3
NCBI Synonym Full Names:nascent polypeptide-associated complex alpha subunit
NCBI Official Symbol:NACA��
NCBI Official Synonym Symbols:HSD48; NACA1; skNAC; NAC-alpha��
NCBI Protein Information:nascent polypeptide-associated complex subunit alpha
UniProt Protein Name:Nascent polypeptide-associated complex subunit alpha, muscle-specific form
UniProt Synonym Protein Names:Alpha-NAC, muscle-specific form; skNAC
Protein Family:Nascent polypeptide-associated complex
UniProt Gene Name:NACA��
UniProt Entry Name:NACAM_HUMAN

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